Lv Zhi-Hua, Ma Pei, Luo Wan, Xiong Hui, Han Lu, Li Si-Wei, Zhou Xin, Tu Jian-Cheng
Department of Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
BMC Cardiovasc Disord. 2014 Nov 14;14:159. doi: 10.1186/1471-2261-14-159.
Free fatty acids (FFAs) play importance roles in the development of diabetes and cardiovascular diseases. We measured serum FFA levels from type 2 diabetes mellitus (T2DM) and acute myocardial infarction (AMI) patients and assay the correlation between serum FFA levels and related factors. The present study was undertaken to investigate a possible relation between the changes in serum free fatty acid concentration with acute myocardial infarction and type 2 diabetes mellitus.
The study population consisted of 540 healthy individuals and 103 patients with T2DM, 59 patients with AMI and 21 volunteers. Serum FFAs were measured with high pressure liquid chromatography. Blood urea nitrogen and uric acid were measured in clinical laboratory, as were glycemic, lipid and blood routine parameters. We selected 242 individuals with age over 60 years, 143 healthy individuals and 52 patients with T2DM, 47 patients with AMI were incorporated into three groups as control group, T2DM group and AMI group. Associations were analyzed with stepwise regression analysis with adjusted for age, sex, body mass index.
Serum FFA levels were significantly higher in the age over 60 years individuals compared to 20 ~ 50 years (logFFA μmmol/L:2.60 ± 0.16 vs. 2.73 ± 0.18, P < .001) in the healthy group. We found lower FFA levels in the AMI compared to the T2DM and control group (2.64 ± 0.22 vs. 2.72 ± 0.13&2.72 ± 0.16, respectively, P < .05&P < 0.01) in the age over 60, fasting blood glucose level higher in the AMI and T2DM (5.78 ± 1.32&7.75 ± 2.93 mmol/L vs. 4.90 ± 0.47 mmol/L, P < .01&P < .001) compared with the normal group, HDL level (1.01 ± 0.22&0.98 ± 0.18 mmol/L vs.1.30 ± 0.22 mmol/L, P < .001&P < .001). With stepwise regression analysis, the serum FFA levels was positively associated with the HDL in the control group (YlogFFA = 2.32 + 0.33XHDL, R = 0.26, P < .01) and T2MD (YlogFFA = 2.46 + 0.27XHDL, R = 0.36, P < .05), AST in AMI (YlogFFA =2.24 + 0. 015XAST, R = 0.49, P < .01).
Compared to control group, serum FFA levels were decreased only in AMI group, while HDL level was increased in both AMI and T2DM group. The serum FFA levels were positive association with the HDL level in both T2DM and control group, FFA levels were positive association with AST in AMI.
游离脂肪酸（FFAs）在糖尿病和心血管疾病的发生发展中起重要作用。我们检测了2型糖尿病（T2DM）患者和急性心肌梗死（AMI）患者的血清FFA水平，并分析血清FFA水平与相关因素之间的相关性。本研究旨在探讨急性心肌梗死和2型糖尿病患者血清游离脂肪酸浓度变化之间的可能关系。
研究人群包括540名健康个体、103名T2DM患者、59名AMI患者和21名志愿者。采用高压液相色谱法测定血清FFAs。在临床实验室检测血尿素氮和尿酸，同时检测血糖、血脂和血常规参数。我们选取242名年龄超过60岁的个体，其中143名健康个体、52名T2DM患者、47名AMI患者被纳入三组，分别作为对照组、T2DM组和AMI组。采用逐步回归分析，并对年龄、性别、体重指数进行校正后分析相关性。
在健康组中，60岁以上个体的血清FFA水平显著高于20至50岁个体（logFFA μmmol/L：2.60±0.16 vs. 2.73±0.18，P<.001）。我们发现，在60岁以上人群中，AMI组的FFA水平低于T2DM组和对照组（分别为2.64±0.22 vs. 2.72±0.13和2.72±0.16，P<.05和P<0.01），AMI组和T2DM组的空腹血糖水平高于正常组（5.78±1.32和7.75±2.93 mmol/L vs. 4.90±0.47 mmol/L，P<.01和P<.001），高密度脂蛋白（HDL）水平低于正常组（1.01±0.22和0.98±0.18 mmol/L vs. 1.30±0.22 mmol/L，P<.001和P<.001）。通过逐步回归分析，对照组（YlogFFA = 2.32 + 0.33XHDL，R = 0.26，P<.01）和T2DM组（YlogFFA = 2.46 + 0.27XHDL，R = 有0.36，P<.05）中血清FFA水平与HDL呈正相关，AMI组中血清FFA水平与谷草转氨酶（AST）呈正相关（YlogFFA =2.24 + 0. 015XAST，R = 0.49，P<.01）。
与对照组相比，仅AMI组血清FFA水平降低，而AMI组和T2DM组的HDL水平均升高。T2DM组和对照组中血清FFA水平与HDL水平呈正相关，AMI组中FFA水平与AST呈正相关。
