Köppen Robert, Bremser Wolfram, Stephan Ina, Klein-Hartwig Karin, Rasenko Tatjana, Koch Matthias
Division 1.7-Food Analysis, Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Str. 11, 12489, Berlin, Germany,
Anal Bioanal Chem. 2015 Apr;407(11):2997-3007. doi: 10.1007/s00216-014-8251-4. Epub 2014 Nov 16.
Trichothecene mycotoxins, with T-2 and HT-2 toxins being the main representatives of the type A subgroup, are naturally and worldwide occurring contaminants frequently found in grain-based food and feed. Due to the high consumption of these products and the potential health risk associated herewith, concerns about the safety and quality of food and feed have increased over the last decades at both governmental and consumer levels. Since it is not possible to avoid their occurrence, tremendous efforts have been performed to identify and monitor mycotoxins in food and feed to make their consumption safe. However, suitable certified reference materials (CRMs) intended for quality assurance and quality control purposes are still lacking for many mycotoxin-matrix combinations. Therefore, in the framework of a European Reference Material (ERM®) project, the first CRM for T-2 and HT-2 toxin in ground oat flakes (ERM®-BC720) was developed according to the requirements of ISO Guide 35. The whole process of ERM®-BC720 development, including sample preparation, homogeneity and stability studies and value assignment, is presented. The assignment of the certified mass fractions was based upon an in-house study using high-performance liquid chromatography isotope-dilution tandem mass spectrometry. Simultaneously, an interlaboratory comparison study involving 24 expert laboratories was conducted in order to support the in-house certification study. The certified values and their corresponding expanded uncertainties (k = 2) for both T-2 and HT-2 toxin in ERM®-BC720, traceable to the international system of units, are (82 ± 4) μg kg(-1) and (81 ± 4) μg kg(-1), respectively.
单端孢霉烯族霉菌毒素以T-2毒素和HT-2毒素为A型亚组的主要代表，是在全球范围内自然存在的污染物，常见于谷物类食品和饲料中。由于这些产品的高消费量以及与之相关的潜在健康风险，在过去几十年里，政府和消费者层面对于食品和饲料的安全性及质量的担忧都有所增加。由于无法避免它们的出现，人们已经付出了巨大努力来识别和监测食品和饲料中的霉菌毒素，以确保其消费安全。然而，对于许多霉菌毒素 - 基质组合，仍然缺乏适用于质量保证和质量控制目的的认证参考物质（CRM）。因此，在一个欧洲参考物质（ERM®）项目框架内，根据ISO指南35的要求，开发了第一种用于燕麦片（ERM®-BC720）中T-2毒素和HT-2毒素的CRM。本文介绍了ERM®-BC720的整个开发过程，包括样品制备、均匀性和稳定性研究以及赋值。认证质量分数的赋值基于一项使用高效液相色谱同位素稀释串联质谱法的内部研究。同时，开展了一项涉及24个专业实验室的实验室间比对研究，以支持内部认证研究。ERM®-BC720中T-2毒素和HT-2毒素的认证值及其相应的扩展不确定度（k = 2），可溯源至国际单位制，分别为(82 ± 4) μg kg⁻¹和(81 ± 4) μg kg⁻¹。
