Popara Marina, Villar Margarita, de la Fuente José
SaBio. Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005, Ciudad Real, Spain.
Methods Mol Biol. 2015;1247:513-27. doi: 10.1007/978-1-4939-2004-4_34.
Ticks are blood-feeding arthropod ectoparasites of wild and domestic animals that transmit disease-causing pathogens to humans and animals worldwide and a good model for the characterization of tick-host-pathogen interactions. Tick-host-pathogen interactions consist of dynamic processes involving genetic traits of hosts, pathogens, and ticks that mediate their development and survival. Proteomics provides information on the protein content of cells and tissues that may differ from results at the transcriptomics level and may be relevant for basic biological studies and vaccine antigen discovery. In this chapter, we describe various methods for protein extraction and for proteomics analysis in ticks based on one-dimensional gel electrophoresis to characterize tick-host-pathogen interactions. Particularly relevant for this characterization is the use of blood-fed ticks. Therefore, we put special emphasis on working with replete ticks collected after feeding on vertebrate hosts.
蜱是野生和家畜的吸血节肢动物外寄生虫，可在全球范围内将致病病原体传播给人类和动物，是蜱-宿主-病原体相互作用特征研究的良好模型。蜱-宿主-病原体相互作用由涉及宿主、病原体和蜱的遗传特征的动态过程组成，这些遗传特征介导它们的发育和存活。蛋白质组学提供有关细胞和组织蛋白质含量的信息，这些信息可能与转录组学水平的结果不同，并且可能与基础生物学研究和疫苗抗原发现相关。在本章中，我们描述了基于一维凝胶电泳的蜱蛋白质提取和蛋白质组学分析的各种方法，以表征蜱-宿主-病原体相互作用。对于这种表征特别相关的是使用饱血蜱。因此，我们特别强调处理以脊椎动物宿主为食后收集的饱血蜱。
