Laboratoire MIVEGEC, UMR CNRS 5290/IRD 224/UM1, Montpellier, France.
Proteomics. 2012 Dec;12(23-24):3510-23. doi: 10.1002/pmic.201200300. Epub 2012 Nov 12.
Vector-borne diseases (VBDs) are defined as infectious diseases of humans and animals caused by pathogenic agents such as viruses, protists, bacteria, and helminths transmitted by the bite of blood-feeding arthropod (BFA) vectors. VBDs represent a major public health threat in endemic areas, generally subtropical zones, and many are considered to be neglected diseases. Genome sequencing of some arthropod vectors as well as modern proteomic and genomic technologies are expanding our knowledge of arthropod-pathogen interactions. This review describes the proteomic approaches that have been used to investigate diverse biological questions about arthropod vectors, including the interplay between vectors and pathogens. Proteomic studies have identified proteins and biochemical pathways that may be involved in molecular crosstalk in BFA-pathogen associations. Future work can build upon this promising start and functional analyses coupled with interactome bioassays will be carried out to investigate the role of candidate peptides and proteins in BFA-human pathogen associations. Dissection of the host-pathogen interactome will be key to understanding the strategies and biochemical pathways used by BFAs to cope with pathogens.
虫媒传染病（VBD）定义为人类和动物的传染病，由病毒、原生动物、细菌和寄生虫等病原体引起，通过吸血节肢动物（BFA）媒介传播。VBD 在流行地区构成重大公共卫生威胁，一般为亚热带地区，其中许多被认为是被忽视的疾病。一些节肢动物媒介的基因组测序以及现代蛋白质组学和基因组学技术正在扩展我们对节肢动物-病原体相互作用的了解。本文综述了用于研究节肢动物媒介的各种生物学问题的蛋白质组学方法，包括媒介与病原体之间的相互作用。蛋白质组学研究鉴定了可能参与 BFA-病原体相关分子串扰的蛋白质和生化途径。未来的工作可以在此有希望的起点上进行，结合功能分析和互作组生物测定，将研究候选肽和蛋白质在 BFA-人类病原体相关中的作用。剖析宿主-病原体互作组将是理解 BFAs 应对病原体的策略和生化途径的关键。
