Dobrzyńska Izabela, Skrzydlewska Elżbieta, Figaszewski Zbigniew A
Institute of Chemistry, University in Białystok, Al. Piłsudskiego 11/4, 15-443, Białystok, Poland.
Department of Analytical Chemistry, Medical University of Białystok, Mickiewicza 2, 15-230, Białystok, Poland.
Cell Biochem Biophys. 2015 Apr;71(3):1517-23. doi: 10.1007/s12013-014-0375-9.
The aim of this study was to determine the influence of cisplatin and novel dinuclear platinum(II) complexes on the membrane electrical properties and lipid peroxidation levels of the Molt-4 human leukemia cell line. Changes in cell function may affect the basal electrical surface properties of cell membranes. These changes can be detected using electrokinetic measurements. Surface charge densities of Molt-4 cells were measured as a function of pH. A four-component equilibrium model was used to describe the interaction between the ions in solution and on cell membrane surfaces. Agreement was found between the experimental and theoretical charge variation curves of the leukemia cells at pH 2.5-9. Lipid peroxidation was estimated by measuring levels of 8-iso-prostaglandine F2α [isoprostanes]. Acid and base functional group concentrations and average association constants with hydroxyl ions were smaller in cisplatin- or dinuclear platinum(II) complex-treated leukemia cell membranes compared to those in untreated cancer cells, and the average association constants with hydrogen ions were higher. Levels of lipid peroxidation products in cisplatin- or dinuclear platinum(II) complex-treated leukemia cell were higher than those found in untreated cancer cells.
本研究的目的是确定顺铂和新型双核铂（II）配合物对Molt-4人白血病细胞系的膜电性质和脂质过氧化水平的影响。细胞功能的变化可能会影响细胞膜的基础电表面性质。这些变化可以通过电动测量来检测。测量了Molt-4细胞的表面电荷密度随pH的变化。使用四组分平衡模型来描述溶液中离子与细胞膜表面离子之间的相互作用。在pH 2.5 - 9时，白血病细胞的实验电荷变化曲线与理论电荷变化曲线之间存在一致性。通过测量8-异前列腺素F2α[异前列腺素]的水平来估计脂质过氧化。与未处理的癌细胞相比，顺铂或双核铂（II）配合物处理的白血病细胞膜中的酸性和碱性官能团浓度以及与氢氧根离子的平均缔合常数较小，而与氢离子的平均缔合常数较高。顺铂或双核铂（II）配合物处理的白血病细胞中脂质过氧化产物的水平高于未处理的癌细胞。
