Coolen S A J, van Buuren B, Duchateau G, Upritchard J, Verhagen H
Unilever Health Institute, Unilever Research & Development Vlaardingen, The Netherlands.
Amino Acids. 2005 Dec;29(4):429-36. doi: 10.1007/s00726-005-0229-2. Epub 2005 Aug 1.
Isoprostanes, non-enzymatic peroxidation products of arachidonic acid, are attractive biomarkers of oxidative stress in research in biology, medicine and nutrition. For the appropriate use of biomarkers it is required that these are both biologically and technically valid. Whereas the biological validity of isoprostanes is well-established, it is technically quite complicated to measure isoprostanes and its metabolites in body fluids, and its rapid disappearance from plasma may hamper practical application. This paper shortly introduces isoprostanes as a biomarker for studies with humans, describes a novel fast and sensitive method for measuring isoprostanes in plasma by high-performance liquid chromatography and tandem mass spectrometry, and provides several examples of the use of the method in studies in humans. By taking care of the biological and technical validity of this biomarker it is possible to establish the antioxidant effects of some food ingredients in studies with human volunteers.
异前列腺素是花生四烯酸的非酶促过氧化产物，在生物学、医学和营养研究中是极具吸引力的氧化应激生物标志物。为了合理使用生物标志物，要求其在生物学和技术层面均有效。虽然异前列腺素的生物学有效性已得到充分证实，但在体液中测量异前列腺素及其代谢物在技术上相当复杂，并且其在血浆中的快速消失可能会妨碍实际应用。本文简要介绍了异前列腺素作为一种用于人体研究的生物标志物，描述了一种通过高效液相色谱和串联质谱法测量血浆中异前列腺素的新型快速灵敏方法，并提供了该方法在人体研究中的几个应用实例。通过关注这种生物标志物的生物学和技术有效性，有可能在人体志愿者研究中确定某些食品成分的抗氧化作用。
