Malolina Ekaterina A, Kulibin Andrey Yu, Kushch Alla A
Ivanovsky Institute of Virology, Ministry of Health of the Russian Federation, Gamaleya str. 16, 123098, Moscow, The Russian Federation.
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str. 26, 119071, Moscow, The Russian Federation.
Reprod Fertil Dev. 2016 Apr;28(6):757-64. doi: 10.1071/RD14255.
Genital tract infection and inflammation may affect male fertility, causing germ and Sertoli cell loss. We determined if testicular cell transplantation is effective at repairing testicular injury induced by herpes simplex virus (HSV) orchitis. ROSA26 mice were used as donors and the recipients were C57BL/6 mice after HSV testicular inoculation; some of the recipients were treated with the antiviral drug acyclovir (ACV). ACV reduced the amount of HSV antigen in testes on Day 3 after transplantation and enhanced the efficacy of transplantation at Day 30. In recipient testes, donor Sertoli cells formed new seminiferous tubules; significantly more new tubules were observed in the testes of ACV-treated mice compared with mice not treated with ACV (17.8% vs 3.6%). Over half (50.4%) of new tubules in ACV-treated testes contained germ cells and round spermatids were detected in 14.2% of new tubules compared with 15.9% and 5.3% in testes not treated with ACV, respectively. At Day 150 the seminiferous epithelium was completely recovered in some donor tubules and elongated spermatids were observed inside it. Thus, our findings reveal the effectiveness of the combination of antiviral therapy with neonatal testis-cell transplantation for the restoration of spermatogenesis damaged by viral infection.
生殖道感染和炎症可能会影响男性生育能力，导致生殖细胞和支持细胞损失。我们确定了睾丸细胞移植对修复单纯疱疹病毒（HSV）睾丸炎所致睾丸损伤是否有效。将ROSA26小鼠作为供体，受体为接种HSV后睾丸的C57BL/6小鼠；部分受体用抗病毒药物阿昔洛韦（ACV）治疗。ACV在移植后第3天减少了睾丸中HSV抗原的量，并在第30天提高了移植效果。在受体睾丸中，供体支持细胞形成了新的生精小管；与未用ACV治疗的小鼠相比，ACV治疗的小鼠睾丸中观察到的新小管明显更多（17.8%对3.6%）。ACV治疗的睾丸中超过一半（50.4%）的新小管含有生殖细胞，14.2%的新小管中检测到圆形精子细胞，而未用ACV治疗的睾丸中这一比例分别为15.9%和5.3%。在第150天，一些供体小管中生精上皮完全恢复，并且在其中观察到了伸长的精子细胞。因此，我们的研究结果揭示了抗病毒治疗与新生睾丸细胞移植相结合对恢复病毒感染损伤的精子发生的有效性。
