蛋白激酶的催化机制与调控
Catalytic mechanisms and regulation of protein kinases.
作者信息
Wang Zhihong, Cole Philip A
机构信息
Department of Chemistry and Biochemistry, University of the Sciences, Philadelphia, Pennsylvania, USA.
Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
出版信息
Methods Enzymol. 2014;548:1-21. doi: 10.1016/B978-0-12-397918-6.00001-X.
Abstract
Protein kinases transfer a phosphoryl group from ATP onto target proteins and play a critical role in signal transduction and other cellular processes. Here, we review the kinase kinetic and chemical mechanisms and their application in understanding kinase structure and function. Aberrant kinase activity has been implicated in many human diseases, in particular cancer. We highlight applications of technologies and concepts derived from kinase mechanistic studies that have helped illuminate how kinases are regulated and contribute to pathophysiology.
摘要
蛋白激酶将磷酸基团从ATP转移到靶蛋白上，在信号转导和其他细胞过程中发挥关键作用。在此，我们综述激酶的动力学和化学机制及其在理解激酶结构与功能方面的应用。异常的激酶活性与许多人类疾病相关，尤其是癌症。我们着重介绍源自激酶机制研究的技术和概念的应用，这些应用有助于阐明激酶是如何被调控的以及如何导致病理生理学变化。
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