具有不对称脂质组成的脂质双分子层膜阵列的高通量形成。
High-throughput formation of lipid bilayer membrane arrays with an asymmetric lipid composition.
作者信息
Watanabe Rikiya, Soga Naoki, Yamanaka Tomoko, Noji Hiroyuki
机构信息
1] Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [2] PRESTO, JST, Bunkyo-ku, Tokyo 113-8656, Japan.
Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan.
出版信息
Sci Rep. 2014 Nov 17;4:7076. doi: 10.1038/srep07076.
We present a micro-device in which more than 10,000 asymmetric lipid bilayer membranes are formed at a time on micro-chamber arrays. The arrayed asymmetric lipid bilayers, where lipid compositions are different between the inner and outer leaflets, are formed with high efficiency of over 97% by injecting several types of liquids into a micro-device that has hydrophilic-in-hydrophobic surfaces. The lipid compositional asymmetry is an intrinsic property of bio-membranes, and therefore, this micro-device extends the versatility of artificial lipid-bilayer systems, which were previously limited to symmetric bilayer formation, and could contribute to the understanding of the role of lipid compositional asymmetry in cell physiology and also to further analytical and pharmacological applications.
我们展示了一种微器件，在该器件的微腔阵列上可一次性形成超过10,000个不对称脂质双层膜。通过将几种类型的液体注入具有亲水-疏水表面的微器件中，可高效形成排列的不对称脂质双层，其内外小叶的脂质组成不同，形成效率超过97%。脂质组成的不对称是生物膜的固有特性，因此，这种微器件扩展了人工脂质双层系统的多功能性，此前该系统仅限于对称双层的形成，并且有助于理解脂质组成不对称在细胞生理学中的作用，以及进一步的分析和药理学应用。
Zotero 插件