体外筛选对溴莠定具有特异性的单链DNA分子识别元件。
In vitro selection of a single-stranded DNA molecular recognition element specific for bromacil.
作者信息
Williams Ryan M, Kulick Amanda R, Yedlapalli Srilakshmi, Battistella Louisa, Hajiran Cyrus J, Sooter Letha J
机构信息
Department of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA ; Memorial Sloan Kettering Cancer Center, Molecular Pharmacology & Chemistry Program, 1275 York Avenue, New York, NY 10065, USA.
Department of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA.
出版信息
J Nucleic Acids. 2014;2014:102968. doi: 10.1155/2014/102968. Epub 2014 Oct 23.
Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (K d = 9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device.
除草定是一种广泛使用的除草剂，已知会污染环境系统。由于其存在的危害以及检测方法效率低下，有必要创建一种快速高效的传感装置。为此，我们利用了一种严格的体外筛选方法来鉴定对除草定具有特异性的单链DNA分子识别元件（MRE）。与筛选的阴性靶标和其他农药相比，我们已鉴定出一种对除草定具有高亲和力（Kd = 9.6 nM）和特异性的MRE。所选择的单链DNA MRE将作为可现场部署的除草定检测装置中的传感元件发挥作用。
Zotero 插件