Dean Kevin M, Fiolka Reto
Opt Express. 2014 Oct 20;22(21):26141-52. doi: 10.1364/OE.22.026141.
Light-sheet fluorescence microscopy (LSFM) affords highly parallelized 3D imaging with optical sectioning capability and minimal light exposure. However, using Gaussian beams for light-sheet generation results in a trade-off between beam waist thickness and the area over which the beam can approximate a light-sheet. Here, we present a novel form of LSFM that uses incoherent extended focusing to produce divergence free light-sheets with near diffraction-limited resolution and uniform intensity distribution along the propagation direction. We demonstrate the imaging performance of the new technique by volumetric imaging of beads, collagen fibers, and melanoma cancer cells with sub-cellular resolution.
光片荧光显微镜（LSFM）能够实现具有光学切片能力且光暴露最小的高度并行化三维成像。然而，使用高斯光束生成光片会在束腰厚度与光束可近似为光片的区域之间产生权衡。在此，我们展示了一种新型的LSFM，它利用非相干扩展聚焦来产生无发散的光片，具有接近衍射极限的分辨率以及沿传播方向均匀的强度分布。我们通过对珠子、胶原纤维和黑色素瘤癌细胞进行亚细胞分辨率的体积成像，展示了这项新技术的成像性能。
