Cheng Siyang, Nakatani Yuya, Gagliano Gabriella, Saliba Nahima, Gustavsson Anna-Karin
Department of Chemistry, Rice University, Houston, TX USA.
Applied Physics Program, Rice University, Houston, TX USA.
Npj Imaging. 2024;2(1):49. doi: 10.1038/s44303-024-00057-9. Epub 2024 Nov 21.
Single-molecule localization microscopy has revealed cellular architectures and molecular dynamics beyond the diffraction limit of light. However, imaging thick samples presents challenges from increased fluorescence background. Light sheet illumination, which utilizes a plane of light for optical sectioning, is effective in reducing fluorescence background, photobleaching, and photodamage. Here, we present the principles of single-molecule localization microscopy and light sheet illumination, followed by an introduction to light sheet microscopy geometries and their imaging applications. Finally, we discuss light sheet illumination approaches for high- and super-resolution imaging of biological structures and dynamics.
单分子定位显微镜揭示了超越光衍射极限的细胞结构和分子动力学。然而，对厚样品成像时，由于荧光背景增加而面临挑战。利用光平面进行光学切片的光片照明在减少荧光背景、光漂白和光损伤方面很有效。在这里，我们介绍单分子定位显微镜和光片照明的原理，接着介绍光片显微镜的几何结构及其成像应用。最后，我们讨论用于生物结构和动力学的高分辨率及超分辨率成像的光片照明方法。
