控制拟南芥高钙反应的数量性状基因座的鉴定。
Identification of quantitative trait loci controlling high Calcium response in Arabidopsis thaliana.
作者信息
Li Wenlong, Duan Huikun, Chen Fengying, Wang Zhi, Huang Xueqing, Deng Xin, Liu Yongxiu
机构信息
Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
出版信息
PLoS One. 2014 Nov 17;9(11):e112511. doi: 10.1371/journal.pone.0112511. eCollection 2014.
Natural variation for primary root growth response to high Ca stress in Arabidopsis thaliana was studied by screening a series of accessions (ecotypes) under high Calcium (40 mM CaCl2) conditions. The genetic basis of this variation was further investigated by QTL analysis using recombinant inbred lines from Landsberg erecta (Ler) × Cape Verde Islands (Cvi) cross. Four QTLs were identified in chromosome 1, 2 and 5,and named response to high Calcium (RHCA) 1-4. The three QTLs (RHCA1, RHCA2 and RHCA4) were further confirmed by analysis of near isogenic lines harboring Cvi introgression fragments in Ler background. Real-time PCR analysis showed that several genes associated with high Ca response including SMT1 and XHT25 have changed expression pattern between Ler and near isogenic lines. These results were useful for detecting molecular mechanisms of plants for high Ca adaption.
通过在高钙（40 mM CaCl₂）条件下筛选一系列拟南芥种质（生态型），研究了拟南芥主根对高钙胁迫生长反应的自然变异。利用来自直立型兰茨贝格（Ler）×佛得角群岛（Cvi）杂交的重组自交系，通过QTL分析进一步研究了这种变异的遗传基础。在第1、2和5号染色体上鉴定出4个QTL，并命名为高钙反应（RHCA）1 - 4。通过对在Ler背景中含有Cvi渗入片段的近等基因系的分析，进一步证实了3个QTL（RHCA1、RHCA2和RHCA4）。实时PCR分析表明，包括SMT1和XHT25在内的几个与高钙反应相关的基因在Ler和近等基因系之间改变了表达模式。这些结果有助于检测植物对高钙适应的分子机制。
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