Ookawa Taiichiro, Hobo Tokunori, Yano Masahiro, Murata Kazumasa, Ando Tsuyu, Miura Hiroko, Asano Kenji, Ochiai Yusuke, Ikeda Mayuko, Nishitani Ryoichi, Ebitani Takeshi, Ozaki Hidenobu, Angeles Enrique R, Hirasawa Tadashi, Matsuoka Makoto
Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
Nat Commun. 2010 Nov 30;1:132. doi: 10.1038/ncomms1132.
The use of fertilizer results in tall rice plants that are susceptible to lodging and results in reduced plant yields. In this study, using chromosome segment substitution lines, we identified an effective quantitative trait loci (QTL) for culm strength, which was designated STRONG CULM2 (SCM2). Positional cloning of the gene revealed that SCM2 was identical to ABERRANT PANICLE ORGANIZATION1 (APO1), a gene previously reported to control panicle structure. A near-isogenic line carrying SCM2 showed enhanced culm strength and increased spikelet number because of the pleiotropic effects of the gene. Although SCM2 is a gain-of-function mutant of APO1, it does not have the negative effects reported for APO1 overexpression mutants, such as decreased panicle number and abnormal spikelet morphology. The identification of lodging-resistance genes by QTL analysis combined with positional cloning is a useful approach for improving lodging resistance and overall productivity in rice.
使用化肥会导致水稻植株长得很高，易倒伏，从而降低作物产量。在本研究中，我们利用染色体片段代换系鉴定出一个控制茎秆强度的有效数量性状位点（QTL），命名为STRONG CULM2（SCM2）。对该基因进行定位克隆后发现，SCM2与ABERRANT PANICLE ORGANIZATION1（APO1）相同，APO1是之前报道的一个控制穗部结构的基因。携带SCM2的近等基因系由于该基因的多效性作用，茎秆强度增强，小穗数增加。尽管SCM2是APO1的功能获得型突变体，但它没有APO1过表达突变体所报道的负面影响，如穗数减少和小穗形态异常。通过QTL分析结合定位克隆来鉴定抗倒伏基因，是提高水稻抗倒伏能力和整体生产力的一种有效方法。
