Toni Cândida, Martos-Sitcha Juan Antonio, Ruiz-Jarabo Ignacio, Mancera Juan Miguel, Martínez-Rodríguez Gonzalo, Pinheiro Carlos Garrido, Heinzmann Berta Maria, Baldisserotto Bernardo
Post-Graduate Program in Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Fish Physiol Biochem. 2015 Feb;41(1):129-38. doi: 10.1007/s10695-014-0011-z. Epub 2014 Nov 18.
This study investigated the effects of prolonged exposure of silver catfish (Rhamdia quelen) to the essential oil (EO) of Hesperozygis ringens. Ventilatory rate (VR), stress and metabolic indicators, energy enzyme activities, and mRNA expression of adenohypophyseal hormones were examined in specimens that were exposed for 6 h to 0 (control), 30 or 50 µL L(-1) EO of H. ringens in water. Reduction in VR was observed in response to each treatment, but no differences were found between treatments. Plasma glucose, protein, and osmolality increased in fish exposed to 50 µL L(-1). Moreover, lactate levels increased after exposure to both EO concentrations. Plasma cortisol levels were not changed by EO exposure. Fish exposed to 30 µL L(-1) EO exhibited higher glycerol-3-phosphate dehydrogenase (G3PDH) activity, while exposure to 50 µL L(-1) EO elicited an increase in glucose-6-phosphate dehydrogenase (G6PDH), fructose-biphosphatase (FBP), and 3-hydroxyacyl-CoA-dehydrogenase (HOAD) activities compared with the control group. Expression of growth hormone (GH) only decreased in fish exposed to 50 µL L(-1) EO, while somatolactin (SL) expression decreased in fish exposed to both concentrations of EO. Exposure to EO did not change prolactin expression. The results indicate that GH and SL are associated with energy reorganization in silver catfish. Fish were only slightly affected by 30 µL L(-1) EO of H. ringens, suggesting that it could be used in practices where a reduction in the movement of fish for prolonged periods is beneficial, i.e., such as during fish transportation.
本研究调查了长吻银鮎（Rhamdia quelen）长时间暴露于环叶香脂树（Hesperozygis ringens）精油（EO）的影响。在水中将样本暴露于0（对照）、30或50 μL L⁻¹环叶香脂树EO中6小时，检测其呼吸频率（VR）、应激和代谢指标、能量酶活性以及腺垂体激素的mRNA表达。每种处理均观察到VR降低，但各处理之间未发现差异。暴露于50 μL L⁻¹的鱼血浆葡萄糖、蛋白质和渗透压升高。此外，暴露于两种EO浓度后乳酸水平均升高。EO暴露未改变血浆皮质醇水平。暴露于30 μL L⁻¹ EO的鱼甘油-3-磷酸脱氢酶（G3PDH）活性较高，而与对照组相比，暴露于50 μL L⁻¹ EO使葡萄糖-6-磷酸脱氢酶（G6PDH）、果糖双磷酸酶（FBP）和3-羟基酰基辅酶A脱氢酶（HOAD）活性增加。仅暴露于50 μL L⁻¹ EO的鱼生长激素（GH）表达降低，而暴露于两种EO浓度的鱼生长抑素（SL）表达均降低。EO暴露未改变催乳素表达。结果表明，GH和SL与长吻银鮎的能量重组有关。长吻银鮎仅受到30 μL L⁻¹环叶香脂树EO的轻微影响，这表明它可用于长时间减少鱼活动有益的实践中，例如在鱼类运输期间。
