Parodi Thaylise V, Cunha Mauro A, Becker Alexssandro G, Zeppenfeld Carla C, Martins Dirlaine I, Koakoski Gessi, Barcellos Leonardo Gil, Heinzmann Berta M, Baldisserotto Bernardo
Programa de Pós Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Fish Physiol Biochem. 2014 Apr;40(2):323-34. doi: 10.1007/s10695-013-9845-z. Epub 2013 Aug 22.
This study investigated the efficacy of the essential oil (EO) of Aloysia triphylla as an anesthetic for albino and gray strains of silver catfish, Rhamdia quelen. Juveniles were exposed to concentrations between 20 and 800 μL L(-1) EO of A. triphylla to evaluate time of induction and recovery from anesthesia. In another experiment, both strains were divided into four groups such as 0 (control), 30, 40, or 50 μL L(-1) EO and transported for 5 h. The longest time for anesthetic induction and recovery was observed in the albinos. Both strains reached anesthesia in the 100-800 μL L(-1) (11.1-1.24 min) range, without mortality, being 200 μL L(-1) the best response considering time to anesthesia (5.35 min). Albinos transported with all EO concentrations showed higher values of carbon dioxide in the water of transport, but lower levels were observed in grays transported with 40 and 50 μL L(-1) EO when compared to control fish. The same concentrations did not prevent significant whole-body cortisol rise at the end of transport in the albino strain. Juveniles of both strains transported with EO presented lower ion loss to the water compared to control fish. The EO of A. triphylla is an effective anesthetic for albino and gray silver catfish. This EO increases whole-body cortisol levels in the albino strain, but as it reduces net ion loss as in the gray strain, it can be also recommended for transport.
本研究调查了三叶轮钟草精油（EO）作为白化和灰色品种银鲶（Rhamdia quelen）麻醉剂的效果。将幼鱼暴露于20至800μL L⁻¹的三叶轮钟草精油浓度下，以评估麻醉诱导时间和苏醒时间。在另一项实验中，将两个品种均分为四组，即0（对照组）、30、40或50μL L⁻¹的精油组，并运输5小时。白化品种的麻醉诱导和苏醒时间最长。两个品种在100 - 800μL L⁻¹（11.1 - 1.24分钟）范围内达到麻醉状态，且无死亡情况，考虑到麻醉时间，200μL L⁻¹是最佳反应浓度（5.35分钟）。所有精油浓度下运输的白化品种在运输水体中的二氧化碳值较高，但与对照鱼相比，40和50μL L⁻¹精油组运输的灰色品种水体中二氧化碳水平较低。相同浓度的精油并不能阻止白化品种在运输结束时全身皮质醇显著升高。与对照鱼相比，用精油运输的两个品种的幼鱼向水中的离子损失较低。三叶轮钟草精油对白化和灰色银鲶是一种有效的麻醉剂。这种精油会使白化品种的全身皮质醇水平升高，但由于它能像灰色品种一样减少净离子损失，因此也可推荐用于运输。
