与牛支原体对四环素敏感性降低相关的16S rRNA基因突变。
16S rRNA gene mutations associated with decreased susceptibility to tetracycline in Mycoplasma bovis.
作者信息
Amram E, Mikula I, Schnee C, Ayling R D, Nicholas R A J, Rosales R S, Harrus S, Lysnyansky I
机构信息
Division of Avian and Aquatic Diseases, Kimron Veterinary Institute, Bet Dagan, Israel Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel.
Division of Avian and Aquatic Diseases, Kimron Veterinary Institute, Bet Dagan, Israel.
出版信息
Antimicrob Agents Chemother. 2015 Feb;59(2):796-802. doi: 10.1128/AAC.03876-14. Epub 2014 Nov 17.
Abstract
Mycoplasma bovis isolates with decreased susceptibilities to tetracyclines are increasingly reported worldwide. The acquired molecular mechanisms associated with this phenomenon were investigated in 70 clinical isolates of M. bovis. Sequence analysis of the two 16S rRNA-encoding genes (rrs3 and rrs4 alleles) containing the primary binding pocket for tetracycline (Tet-1 site) was performed on isolates with tetracycline hydrochloride MICs of 0.125 to 16 μg/ml. Mutations at positions A965T, A967T/C (Escherichia coli numbering) of helix 31, U1199C of helix 34, and G1058A/C were identified. Decreased susceptibilities to tetracycline (MICs, ≥2 μg/ml) were associated with mutations present at two (A965 and A967) or three positions (A965, A967, and G1058) of the two rrs alleles. No tet(M), tet(O), or tet(L) determinants were found in the genome of any of the 70 M. bovis isolates. The data presented correlate (P<0.0001) the mutations identified in the Tet-1 site of clinical isolates of M. bovis with decreased susceptibility to tetracycline.
摘要
全球范围内，对四环素敏感性降低的牛支原体分离株的报道日益增多。我们对70株牛支原体临床分离株进行研究，以探究与此现象相关的获得性分子机制。对盐酸四环素最低抑菌浓度（MIC）为0.125至16μg/ml的分离株，进行了包含四环素主要结合位点（Tet-1位点）的两个16S rRNA编码基因（rrs3和rrs4等位基因）的序列分析。在31螺旋的A965T、A967T/C（大肠杆菌编号）位置、34螺旋的U1199C以及G1058A/C处发现了突变。对四环素敏感性降低（MIC≥2μg/ml）与两个rrs等位基因中两个位置（A965和A967）或三个位置（A965、A967和G1058）出现的突变有关。在70株牛支原体分离株的基因组中均未发现tet(M)、tet(O)或tet(L)决定簇。所呈现的数据表明（P<0.0001），牛支原体临床分离株Tet-1位点的突变与对四环素敏感性降低相关。
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