Abarikwu Sunny O, Akiri Oghenetega F, Durojaiye Mojisola A, Adenike Alabi
Department of Biochemistry, University of Port Harcourt , Choba , Nigeria and.
Toxicol Mech Methods. 2015 Jan;25(1):70-80. doi: 10.3109/15376516.2014.989349.
The potential toxicity resulting from the possible interactions of the herbicides, Ultrazin (atrazine, ATZ) and Bretmont Wipeout (glyphosate, GLY) (as commercialized in Nigeria), is not completely known. We therefore evaluated reproductive- and hepato-toxicity in rats co-exposed to ATZ and GLY.
Six weeks old male rats were exposed by gavage three times per week to ATZ (12.5 mg/kg) or GLY (5 mg/kg) alone or in combination (12.5 mg/kg ATZ + 5 mg/kg GLY) or vehicle (corn oil), for 52 days.
ATZ and GLY impaired sperm quality but GLY has more adverse effect on sperm quality than ATZ. Testosterone level, sperm motility, sperm counts, live/dead ratio and the weight of the epididymis were lower in the GLY group compared to the ATZ group by 57%, 33%, 20%, 22% and 41% and higher by 109%, 76.7%, 39.6%, 32.3% and 100% respectively in the combine-exposure group (ATZ + GLY) compared to the GLY group. Oxidative stress and histopathological changes were also noticeable in the liver but not in the testis of GLY-treated animals, and the observed effects were more remarkable in the GLY group than the ATZ or the combined-exposure group. The combined effects of the active ingredients on testosterone level, sperm count and hepatic malondialdehyde (MDA) levels were also similar as when the commercial formulations were used.
There are therefore antagonistic interactions between the two toxicants on the toxicity endpoints investigated in this study and these effects are due to the active ingredients of both herbicides in the commercial formulations.
除草剂“超嗪”（莠去津，ATZ）和“布雷蒙特灭草剂”（草甘膦，GLY）（尼日利亚商业化产品）之间可能的相互作用所产生的潜在毒性尚不完全清楚。因此，我们评估了同时暴露于ATZ和GLY的大鼠的生殖毒性和肝毒性。
六周龄雄性大鼠每周经口灌胃三次，分别给予单独的ATZ（12.5毫克/千克）或GLY（5毫克/千克）或二者组合（12.5毫克/千克ATZ + 5毫克/千克GLY）或溶剂（玉米油），持续52天。
ATZ和GLY均损害精子质量，但GLY对精子质量的不良影响比ATZ更大。与ATZ组相比，GLY组的睾酮水平、精子活力、精子计数、活/死比率和附睾重量分别降低了57%、33%、20%、22%和41%；与GLY组相比，联合暴露组（ATZ + GLY）的上述指标分别升高了109%、76.7%、39.6%、32.3%和100%。氧化应激和组织病理学变化在GLY处理动物的肝脏中也很明显，但在睾丸中未观察到，并且GLY组观察到的影响比ATZ组或联合暴露组更显著。活性成分对睾酮水平、精子计数和肝脏丙二醛（MDA）水平的联合作用与使用商业制剂时相似。
因此，在本研究中所调查的毒性终点方面，这两种毒物之间存在拮抗相互作用，且这些影响是由于商业制剂中两种除草剂的活性成分所致。
