Salles B, Weinstock G M
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225.
Mol Gen Genet. 1989 Feb;215(3):537-42. doi: 10.1007/BF00427053.
Analysis of the induction of expression of cea-lacZ fusions in cya and crp mutants showed that catabolite repression affects the kinetics of induction and the rate of induced synthesis. In a cya mutant, addition of cAMP reduced the induction lag and increased the amount of beta-galactosidase produced. The CRP-cAMP complex was found to bind to two sites 5' to the cea promoter, but deletion analysis showed that only one of these was involved in the control of cea. Deletion of this site resulted in a loss of the stimulatory effects of cAMP in a cya mutant.
对cya和crp突变体中cea-lacZ融合基因表达诱导的分析表明，分解代谢物阻遏影响诱导动力学和诱导合成速率。在cya突变体中，添加cAMP可缩短诱导延迟并增加β-半乳糖苷酶的产生量。发现CRP-cAMP复合物结合在cea启动子5'端的两个位点，但缺失分析表明其中只有一个位点参与cea的调控。缺失该位点导致cya突变体中cAMP的刺激作用丧失。
