Cardiff School of Health Sciences, Cardiff Metropolitan University, Western Avenue, Llandaff, Cardiff CF5 2YB, Wales, UK.
Cardiff School of Health Sciences, Cardiff Metropolitan University, Western Avenue, Llandaff, Cardiff CF5 2YB, Wales, UK
J Antimicrob Chemother. 2015 Mar;70(3):716-25. doi: 10.1093/jac/dku448. Epub 2014 Nov 16.
Manuka honey is a broad-spectrum antimicrobial agent that seems to affect different bacteria in many different ways. It has been shown to be bactericidal against Pseudomonas aeruginosa by destabilizing the cell wall, but we aimed to investigate whether there were further intracellular target sites.
In this study inhibitory effects of manuka honey on P. aeruginosa were investigated using hydrophobicity assays, two-dimensional electrophoresis, quantitative RT-PCR, transmission electron microscopy and motility assays.
Exposure of P. aeruginosa to manuka honey reduced both swarming and swimming motility. Moreover, this was a consequence of de-flagellation of the bacterial cell, which was correlated with decreased expression of the major structural flagellin protein, FliC, and concurrent suppression of flagellin-associated genes, including fliA, fliC, flhF, fleN, fleQ and fleR. The differential expression of the flagellar regulon in the presence of manuka honey was mapped schematically. Flagella are integral to bacterial adhesion, the initiation of infection and biofilm formation, and swarming has been associated with increased virulence.
By limiting motility in vitro, we infer that manuka honey impacts on the virulence of P. aeruginosa. This deduction must now be tested in vivo.
麦卢卡蜂蜜是一种广谱抗菌剂，似乎以多种不同的方式影响不同的细菌。已证实它通过破坏细胞壁对铜绿假单胞菌具有杀菌作用，但我们旨在研究是否存在其他细胞内靶位。
在这项研究中，使用疏水性测定、二维电泳、定量 RT-PCR、透射电子显微镜和泳动试验研究了麦卢卡蜂蜜对铜绿假单胞菌的抑制作用。
暴露于麦卢卡蜂蜜中的铜绿假单胞菌减少了群集运动和游泳运动。此外，这是细菌细胞鞭毛脱落的结果，与主要结构鞭毛蛋白 FliC 的表达降低以及鞭毛相关基因（包括 fliA、fliC、flhF、fleN、fleQ 和 fleR）的同时抑制相关。麦卢卡蜂蜜存在时，flagellar regulon 的差异表达以示意图形式表示。鞭毛是细菌黏附、感染起始和生物膜形成的组成部分，群集运动与毒力增加有关。
通过限制体外运动，我们推断麦卢卡蜂蜜会影响铜绿假单胞菌的毒力。现在必须在体内进行这一推断的验证。
