Lindstein T, June C H, Ledbetter J A, Stella G, Thompson C B
Howard Hughes Medical Institute, University of Michigan, Ann Arbor 48109.
Science. 1989 Apr 21;244(4902):339-43. doi: 10.1126/science.2540528.
Quiescent T cells can be induced to express many genes by mitogen or antigen stimulation. The messenger RNAs of some of these genes undergo relatively rapid degradation compared to messenger RNAs from constitutively expressed genes. A T cell activation pathway that specifically regulates the stability of messenger RNAs for the lymphokines interleukin-2, interferon-gamma, tumor necrosis factor-alpha, and granulocyte-macrophage colony-stimulating factor is induced by stimulation of the CD28 surface molecule. This pathway does not directly affect the steady-state messenger RNA level, transcription, or messenger RNA half-life of other T cell activation genes, including c-myc, c-fos, IL-2 receptor, and the 4F2HC surface antigen. These data show that stimuli received at the cell surface can alter gene expression by inducing specific changes in messenger RNA degradation.
静止的T细胞可通过丝裂原或抗原刺激被诱导表达许多基因。与组成性表达基因的信使RNA相比，其中一些基因的信使RNA降解相对较快。CD28表面分子的刺激可诱导一种T细胞激活途径，该途径特异性调节白细胞介素-2、干扰素-γ、肿瘤坏死因子-α和粒细胞-巨噬细胞集落刺激因子等淋巴因子信使RNA的稳定性。该途径不会直接影响其他T细胞激活基因的稳态信使RNA水平、转录或信使RNA半衰期，这些基因包括c-myc、c-fos、IL-2受体和4F2HC表面抗原。这些数据表明，细胞表面接收到的刺激可通过诱导信使RNA降解的特定变化来改变基因表达。
