Ogden Samantha J, Horton Jeffrey K, Stubbs Simon L, Tatnell Peter J
GE Healthcare Life Sciences R&D, The Maynard Centre, Forest Farm, Whitchurch, Cardiff, CF14 7YT, U.K.
J Forensic Sci. 2015 Jan;60 Suppl 1:S207-12. doi: 10.1111/1556-4029.12622. Epub 2014 Nov 18.
The 1.2 mm Electric Coring Tool (e-Core™) was developed to increase the throughput of FTA(™) sample collection cards used during forensic workflows and is similar to a 1.2 mm Harris manual micro-punch for sampling dried blood spots. Direct short tandem repeat (STR) DNA profiling was used to compare samples taken by the e-Core tool with those taken by the manual micro-punch. The performance of the e-Core device was evaluated using a commercially available PowerPlex™ 18D STR System. In addition, an analysis was performed that investigated the potential carryover of DNA via the e-Core punch from one FTA disc to another. This contamination study was carried out using Applied Biosystems AmpflSTR™ Identifiler™ Direct PCR Amplification kits. The e-Core instrument does not contaminate FTA discs when a cleaning punch is used following excision of discs containing samples and generates STR profiles that are comparable to those generated by the manual micro-punch.
1.2毫米电动取芯工具（e-Core™）的开发旨在提高法医工作流程中使用的FTA(™)样本采集卡的通量，它类似于用于采集干血斑的1.2毫米哈里斯手动微型打孔器。直接短串联重复序列（STR）DNA分析用于比较e-Core工具采集的样本与手动微型打孔器采集的样本。使用市售的PowerPlex™ 18D STR系统评估e-Core设备的性能。此外，还进行了一项分析，研究DNA通过e-Core打孔器从一个FTA圆盘转移到另一个圆盘的潜在残留情况。这项污染研究使用了应用生物系统公司的AmpflSTR™ Identifiler™直接PCR扩增试剂盒。当在切除含有样本的圆盘后使用清洁打孔器时，e-Core仪器不会污染FTA圆盘，并且生成的STR图谱与手动微型打孔器生成的图谱相当。
