Wajid Nadia, Naseem Rashida, Anwar Sanam Saiqa, Awan Sana Javaid, Ali Muhammad, Javed Sara, Ali Fatima
IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan,
Cell Tissue Bank. 2015 Sep;16(3):389-97. doi: 10.1007/s10561-014-9483-4. Epub 2014 Nov 19.
Stomal cells derived from Wharton's jelly of human umbilical cord (WJMSCs) are considered as the potential therapeutic agents for regeneration and are getting famous for stem cell banking. Our study aims to evaluate the effects of gestational diabetes on proliferation capacity and viability of WJMSCs. Mesenchymal stromal cells were isolated from Wharton's jelly of human umbilical cords from normal and gestational diabetic (DWJMSCs) mothers. Growth patterns of both types of cells were analyzed through MTT assay and population doubling time. Cell survival, cell death and glucose utilization were estimated through trypan blue exclusion assay, LDH assay and glucose detection assay respectively. Angiogenic ability was evaluated by immunocytochemistry and ELISA for VEGF A. Anti-cancerous potential was analyzed on HeLa cells. DWJMSCs exhibited low proliferative rate, increased population doubling time, reduced cell viability and increased cell death. Interestingly, DWJMSCs were found to have a reduced glucose utilization and anti-cancerous ability while enhanced angiogenic ability. Gestational diabetes induces adverse effects on growth, angiogenic and anti-cancerous potential of WJMSCs.
源自人脐带华通氏胶的间充质基质细胞（WJMSCs）被视为再生的潜在治疗剂，并因干细胞库而闻名。我们的研究旨在评估妊娠期糖尿病对WJMSCs增殖能力和活力的影响。从正常母亲和妊娠期糖尿病母亲（DWJMSCs）的脐带华通氏胶中分离间充质基质细胞。通过MTT法和群体倍增时间分析两种细胞的生长模式。分别通过台盼蓝排斥试验、乳酸脱氢酶（LDH）试验和葡萄糖检测试验评估细胞存活、细胞死亡和葡萄糖利用情况。通过免疫细胞化学和VEGF A的酶联免疫吸附测定（ELISA）评估血管生成能力。在HeLa细胞上分析抗癌潜力。DWJMSCs表现出低增殖率、群体倍增时间增加、细胞活力降低和细胞死亡增加。有趣的是，发现DWJMSCs的葡萄糖利用和抗癌能力降低，而血管生成能力增强。妊娠期糖尿病对WJMSCs的生长、血管生成和抗癌潜力产生不利影响。
