School of Veterinary Medicine, Ceará State University (UECE), Fortaleza, CE, 60.714-903, Brazil.
School of Veterinary Medicine, Federal University of Rio Grande Do Sul (UFRGS), Porto Alegre, RS, 90040-060, Brazil.
Vet Res Commun. 2022 Feb;46(1):169-182. doi: 10.1007/s11259-021-09843-x. Epub 2021 Oct 9.
Mesenchymal stem cells (MSCs) from the umbilical cord (UC) have aroused considerable interest. However, little is known about the maternal effect on these cells. The aim of this study was to verify the impact of the nutritional status of donor goats on the growth and differentiation of MSCs from the UC. At parturition, 19 goats were grouped based on their low or high body mass index (low BMI, LBMI, n = 9; and high BMI, HBMI, n = 10). UCs were collected during delivery and Wharton's jelly (WJ) fragments cultured. WJ-MSCs were differentiated into osteocytes, adipocytes, chondrocytes, and the population doubling time (PDT) was determined. Samples of WJ-MSCs were also used to verify the expression of the CD90, CD73, CD34, CD45, and CD105 genes. Media used for WJ-MSC primary cultures were analyzed using near-infrared spectroscopy. The lag phase was 7.5 ± 0.6 days and the entire culture took 26.7 ± 1.3 days, with a cell proliferation rate of 8.500 cells/day. The mean PDT from subculture was 30.0 ± 0.7 h. The CD105 gene was sub-expressed in LBMI, and the spectra of the spent media from the second to fourth day of WJ-MSC primary culture were segregated into negative scores by multivariate analysis. We conclude that, in goats, the nutritional balance of the donor did not affect the in vitro growth of MSCs derived from the UC. However, the molecular profile observed in the low BMI group suggests that the use of MSCs for therapeutic purposes should be considered more carefully.
脐带间充质干细胞（MSCs）引起了相当大的兴趣。然而，人们对这些细胞的母体效应知之甚少。本研究旨在验证供体山羊的营养状况对脐带间充质干细胞生长和分化的影响。在分娩时，根据低或高体重指数（低 BMI，LBMI，n = 9；高 BMI，HBMI，n = 10）将 19 只山羊分组。在分娩过程中收集脐带，并培养沃顿胶（WJ）碎片。将 WJ-MSCs 分化为成骨细胞、脂肪细胞、软骨细胞，并确定倍增时间（PDT）。还使用 WJ-MSCs 样本来验证 CD90、CD73、CD34、CD45 和 CD105 基因的表达。使用近红外光谱分析用于 WJ-MSC 原代培养的培养基。潜伏期为 7.5±0.6 天，整个培养时间为 26.7±1.3 天，细胞增殖率为 8.500 个细胞/天。传代后的平均 PDT 为 30.0±0.7 小时。LBMI 中 CD105 基因表达下调，多元分析将 WJ-MSC 原代培养第 2 至第 4 天的耗竭培养基的光谱分为负分数。我们得出结论，在山羊中，供体的营养平衡不会影响从 UC 中分离的 MSC 的体外生长。然而，在低 BMI 组中观察到的分子谱表明，应更仔细地考虑将 MSCs 用于治疗目的。
