Eber Fabian J, Eiben Sabine, Jeske Holger, Wege Christina
Department of Molecular Biology and Plant Virology, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, D-70569 Stuttgart, Germany.
Nanoscale. 2015 Jan 7;7(1):344-55. doi: 10.1039/c4nr05434b.
The in vitro assembly of artificial nanotubular nucleoprotein shapes based on tobacco mosaic virus-(TMV-)-derived building blocks yielded different spatial organizations of viral coat protein subunits on genetically engineered RNA molecules, containing two or multiple TMV origins of assembly (OAs). The growth of kinked nanoboomerangs as well as of branched multipods was determined by the encapsidated RNAs. A largely simultaneous initiation at two origins and subsequent bidirectional tube elongation could be visualized by transmission electron microscopy of intermediates and final products. Collision of the nascent tubes' ends produced angular particles with well-defined arm lengths. RNAs with three to five OAs generated branched multipods with a maximum of four arms. The potential of such an RNA-directed self-assembly of uncommon nanotubular architectures for the fabrication of complex multivalent nanotemplates used in functional hybrid materials is discussed.
基于烟草花叶病毒（TMV）衍生构建块的人工纳米管状核蛋白形状的体外组装，在含有两个或多个TMV组装起始位点（OAs）的基因工程RNA分子上产生了病毒衣壳蛋白亚基的不同空间组织。带纽纳米回飞棒和分支多脚架的生长由包封的RNA决定。通过对中间体和最终产物的透射电子显微镜观察，可以看到在两个起始位点基本同时起始并随后双向管延伸。新生管末端的碰撞产生了具有明确臂长的角状颗粒。具有三到五个OAs的RNA产生了最多四个臂的分支多脚架。本文讨论了这种RNA定向自组装罕见纳米管状结构在制造用于功能杂化材料的复杂多价纳米模板方面的潜力。
