Jang Hun-Jae, Hur Eugene, Kim Yoonkwan, Lee Seol-Hoon, Kang Nae G, Yoh Jack J
Seoul National University, Department of Mechanical and Aerospace Engineering, 1 Gwanakro, Gwanakgu, Seoul 151-742, Republic of Korea.
LG Household & Health Care R&D Center, 84 Jang-dong, Yuseong-gu, Daejeon 305-343, Republic of Korea.
J Biomed Opt. 2014 Nov;19(11):118002. doi: 10.1117/1.JBO.19.11.118002.
A breakthrough in the efficient transdermal delivery of drug via the laser-driven microjet is reported. A single source of laser beam is split into two: one beam ablates a targeted spot on a skin and another beam drives the injector for fast microjet ejection into a preablated spot. This combined ablation and microjet injection scheme using a beam splitter utilizes laser energy sharing between generation of the microhole via ablation and the microjet which is generated using the Er:YAG laser beam at a 2940-nm wavelength and pulse duration. A careful analysis of the injection mechanism is carried out by studying the response of the elastic membrane that separates a driving water unit for bubble expansion from a drug unit for a microjet ejection. The efficiency of the present delivery scheme is evaluated by the abdominal porcine skin test using the fluorescein isothiocyanate staining and the confocal microscopy for quantitative delivery confirmation. The depth of penetration and the injected volume of the drug are also confirmed by polyacrylamide gel tests.
据报道，通过激光驱动微射流实现药物高效透皮递送方面取得了一项突破。单一激光束源被分成两束：一束光束消融皮肤上的目标部位，另一束光束驱动注射器，以便将微射流快速喷射到预先消融的部位。这种使用分束器的联合消融和微射流注射方案，利用了通过消融产生微孔与使用波长为2940纳米、脉冲持续时间的铒钇铝石榴石激光束产生微射流之间的激光能量共享。通过研究将用于气泡膨胀的驱动水单元与用于微射流喷射的药物单元分隔开的弹性膜的响应，对注射机制进行了仔细分析。通过使用异硫氰酸荧光素染色的猪腹部皮肤试验和用于定量递送确认的共聚焦显微镜，评估了当前递送方案的效率。还通过聚丙烯酰胺凝胶试验确认了药物的渗透深度和注射体积。
