在乌克兰分离出的两株特里贝奇病毒的特性研究。

Characterization of two strains of Tribeč virus isolated in Ukraine.

作者信息

Dedkov Vladimir G, Dubina Dmitriy A, Yurchenko Oksana A, Bekova Marina V, Valdokhina Anna V, Shipulin German A

机构信息

1 Central Research Institute for Epidemiology , Russian Inspectorate for Protection of Consumer Rights and Human Welfare, Moscow, Russia .

出版信息

Vector Borne Zoonotic Dis. 2014 Nov;14(11):808-16. doi: 10.1089/vbz.2014.1683.

DOI:10.1089/vbz.2014.1683

PMID:25409272

Abstract

During arbovirus surveillance in Ixodes ricinus ticks in South Ukraine, two strains of Tribeč virus were isolated using an in vivo method and characterized using the complement fixation text (CFT), hemagglutination assay (HA), electron microscopy, and molecular methods. Both strains replicated well in the BHK-21, PEC, and Vero cell lines and demonstrated 95% nucleotide identity in their VP3 sequences compared with the reference VP3 sequence for Tribeč virus. These two strains of Tribeč virus were named Tr35 and Tr19. Also, segment reassortment involving Tr35, Tr19, TRBV, and LIPV strains was shown.

摘要

在乌克兰南部蓖麻硬蜱的虫媒病毒监测期间，使用体内方法分离出两株特里贝奇病毒，并通过补体结合试验（CFT）、血凝试验（HA）、电子显微镜和分子方法进行了鉴定。两株病毒在BHK-21、PEC和Vero细胞系中均能良好复制，与特里贝奇病毒的参考VP3序列相比，其VP3序列的核苷酸同一性为95%。这两株特里贝奇病毒分别命名为Tr35和Tr19。此外，还显示了涉及Tr35、Tr19、TRBV和LIPV毒株的片段重配。