Herrin Brian H, Zajac Anne M, Little Susan E
1 Veterinary Pathobiology, Oklahoma State University Center for Veterinary Health Sciences , Stillwater, Oklahoma.
Vector Borne Zoonotic Dis. 2014 Nov;14(11):821-3. doi: 10.1089/vbz.2014.1661.
To determine the prevalence of Borrelia burgdorferi and Anaplasma phagocytophilum in a newly established population of Ixodes scapularis in the mountainous region of southwestern Virginia, questing adult ticks were collected and the identity and infection status of each tick was confirmed by PCR and sequencing. A total of 364 adult ticks were tested from three field sites. B. burgdorferi sensu stricto was identified in a total of 32/101 (32%) ticks from site A, 49/154 (32%) ticks from site B, and 36/101 (36%) ticks from site C, for a total prevalence rate of 33% (117/356). In addition, A. phagocytophilum was detected in 3/364 (0.8%) ticks, one from site A and two from site B. The prevalence of both pathogens in ticks at these sites is similar to that reported from established endemic areas. These data document the presence of I. scapularis and the agent of Lyme disease in a newly established area of the Appalachian region, providing further evidence of range expansion of both the tick and public and veterinary health risk it creates.
为确定弗吉尼亚州西南部山区新建立的肩突硬蜱种群中伯氏疏螺旋体和嗜吞噬细胞无形体的流行情况，采集了正在 questing 的成年蜱，并通过 PCR 和测序确认了每只蜱的身份和感染状况。从三个野外地点共检测了 364 只成年蜱。在来自地点 A 的 101 只蜱中有 32 只（32%）、来自地点 B 的 154 只蜱中有 49 只（32%）以及来自地点 C 的 101 只蜱中有 36 只（36%）检测到狭义伯氏疏螺旋体，总流行率为 33%（117/356）。此外，在 364 只蜱中有 3 只（0.8%）检测到嗜吞噬细胞无形体，1 只来自地点 A，2 只来自地点 B。这些地点蜱中两种病原体的流行率与已建立的流行地区报告的相似。这些数据证明了阿巴拉契亚地区新建立区域中存在肩突硬蜱和莱姆病病原体，为蜱的分布范围扩大以及它所带来的公共和兽医健康风险提供了进一步证据。 （注：原文中“questing”这个词在医学蜱类相关语境中，可能是指蜱寻找宿主的行为，但这个词在这里不太好准确翻译，暂保留英文）
