Onodera Tomoko, Futai Eugene, Kan Eiichiro, Abe Naoki, Uchida Takafumi, Kamio Yoshiyuki, Kaneko Jun
Department of Microbial Biotechnology and Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Sendai 981-8555, Japan; and Department of Human Health and Nutrition, Graduate School of Comprehensive Human Sciences, Shokei Gakuin University, Yurigaoka 4-10-1, Natori 981-1295, Japan.
Department of Microbial Biotechnology and Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Sendai 981-8555, Japan; and Department of Human Health and Nutrition, Graduate School of Comprehensive Human Sciences, Shokei Gakuin University, Yurigaoka 4-10-1, Natori 981-1295, Japan
J Biochem. 2015 May;157(5):301-9. doi: 10.1093/jb/mvu074. Epub 2014 Nov 18.
Plasmalogens (Pls) are widely distributed in the biological membrane of animals and certain anaerobic bacteria, but their functions in the cell membrane are still poorly understood. Decrease of phosphatidylethanolamine plasmalogen (PEPls) in the brain tissue of patients with Alzheimer's disease prompted us to investigate the effect of the membrane phosphorus lipid composition on the activity of γ-secretase that produces amyloid-beta protein (Aβ). To clarify the effect of phospholipids, including PEPls, on Aβ production, γ-secretase activity was measured in an in vitro assay using yeast microsomes and reconstituted liposomes. The presence of ethanolamine phospholipids in the proteoliposome weakened γ-secretase activity. In addition, increased PEPls content in total ethanolamine phospholipids further decreased the enzyme activity, indicating that γ-secretase activity is affected by the membrane phospholipid PEPls/PE ratio. Furthermore, PEPls from anaerobic bacterial cell membrane induced the same effect on γ-secretase activity.
缩醛磷脂广泛分布于动物和某些厌氧细菌的生物膜中，但其在细胞膜中的功能仍知之甚少。阿尔茨海默病患者脑组织中磷脂酰乙醇胺缩醛磷脂（PEPls）的减少促使我们研究膜磷脂组成对产生β淀粉样蛋白（Aβ）的γ-分泌酶活性的影响。为了阐明包括PEPls在内的磷脂对Aβ产生的影响，在使用酵母微粒体和重组脂质体的体外试验中测量了γ-分泌酶活性。蛋白脂质体中乙醇胺磷脂的存在会削弱γ-分泌酶活性。此外，总乙醇胺磷脂中PEPls含量的增加进一步降低了酶活性，表明γ-分泌酶活性受膜磷脂PEPls/PE比例的影响。此外，来自厌氧细菌细胞膜的PEPls对γ-分泌酶活性也有相同的影响。
