Calahorra M, Opekarová M, Ramirez J, Peña A
Department of Microbiology, Universidad Nacional Autónoma de México, Mexico City.
FEBS Lett. 1989 Apr 24;247(2):235-8. doi: 10.1016/0014-5793(89)81342-0.
Yeast plasma membrane vesicles were obtained by the fusion of liposomes with purified yeast membranes by means of the freeze thaw-sonication technique. Beef heart mitochondria cytochrome-c oxidase was incorporated into the vesicles. Addition of substrate (ascorbate/TMPD/cytochrome c) generated a membrane potential negative inside, and an alkaline pH gradient inside the vesicle, that served as the driving force for leucine transport. Both delta pH and delta psi could drive leucine transport. When delta pH was increased in the presence of valinomycin and potassium, at the expense of delta psi, leucine uptake increased by 10%.
通过冻融-超声处理技术使脂质体与纯化的酵母膜融合，从而获得酵母质膜囊泡。将牛心线粒体细胞色素c氧化酶整合到囊泡中。添加底物（抗坏血酸/四甲基对苯二胺/细胞色素c）会产生膜内负电位以及囊泡内的碱性pH梯度，这作为亮氨酸转运的驱动力。ΔpH和Δψ都可以驱动亮氨酸转运。当在缬氨霉素和钾存在的情况下增加ΔpH时，以Δψ为代价，亮氨酸摄取增加了10%。
