在添加辛酸的培养基中，酿酒酵母质膜中的H(+)-ATP酶在生长潜伏期被激活，同时细胞内pH值和细胞活力下降。
The H(+)-ATPase in the plasma membrane of Saccharomyces cerevisiae is activated during growth latency in octanoic acid-supplemented medium accompanying the decrease in intracellular pH and cell viability.
作者信息
Viegas C A, Almeida P F, Cavaco M, Sá-Correia I
机构信息
Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Lisbon, Portugal.
出版信息
Appl Environ Microbiol. 1998 Feb;64(2):779-83. doi: 10.1128/AEM.64.2.779-783.1998.
Abstract
Saccharomyces cerevisiae plasma membrane H(+)-ATPase activity was stimulated during octanoic acid-induced latency, reaching maximal values at the early stages of exponential growth. The time-dependent pattern of ATPase activation correlated with the decrease of cytosolic pH (pHi). The cell population used as inoculum exhibited a significant heterogeneity of pHi, and the fall of pHi correlated with the loss of cell viability as determined by plate counts. When exponential growth started, only a fraction of the initial population was still viable, consistent with the role of the physiology and number of viable cells in the inoculum in the duration of latency under acid stress.
摘要
在辛酸诱导的潜伏期内，酿酒酵母质膜H(+) -ATP酶活性受到刺激，在指数生长早期达到最大值。ATP酶激活的时间依赖性模式与胞质pH（pHi）的降低相关。用作接种物的细胞群体表现出pHi的显著异质性，并且pHi的下降与平板计数所确定的细胞活力丧失相关。当指数生长开始时，只有初始群体的一部分仍然存活，这与接种物中活细胞的生理学和数量在酸胁迫下潜伏期持续时间中的作用一致。
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