Morosoli R, Durand S, Boucher F
Centre de recherche en microbiologie appliquée, Institut Armand-Frappier, Québec, Canada.
FEMS Microbiol Lett. 1989 Jan 1;48(1):57-60. doi: 10.1016/0378-1097(89)90146-8.
Cryptococcus albidus secretes a xylanase when induced by xylan or beta-methylxyloside, a non-metabolizable inducer, and production of the enzyme is repressed by xylose. The effect of exogenous cAMP on xylanase production was tested under different growth conditions. The cAMP elicited a 1.5 to 2 fold increase in xylanase production during the induction by xylan and B-methylxyloside but did not relieve the repression observed during growth on xylose. Cyclic AMP also affected the growth rate of the cells and did not modulate the activity of pure xylanase in vitro. A 15-nucleotide sequence located upstream from the xylanase gene could be part of a cAMP regulatory sequence.
浅白隐球菌在木聚糖或β-甲基木糖苷（一种不可代谢的诱导剂）诱导下会分泌木聚糖酶，且木糖会抑制该酶的产生。在不同生长条件下测试了外源环磷酸腺苷（cAMP）对木聚糖酶产生的影响。在木聚糖和β-甲基木糖苷诱导过程中，cAMP使木聚糖酶的产量提高了1.5至2倍，但并未解除在木糖上生长时所观察到的抑制作用。环磷酸腺苷还影响细胞的生长速率，且在体外不会调节纯木聚糖酶的活性。木聚糖酶基因上游的一段15个核苷酸序列可能是cAMP调控序列的一部分。
