Cha Injun, Kim Nan-Ok, Nam Jung-Gu, Choi Eun-Suk, Chung Gyung Tae, Kang Yeon-Ho, Hong Sahyun
Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health.
Jpn J Infect Dis. 2014;67(6):490-4. doi: 10.7883/yoken.67.490.
Forty domestic and travel-associated Campylobacter jejuni isolates were analyzed by profiling 7 pathogenic genes (cdtB, cadF, Cj0131, ciaB, racR, wlaN, and virB11) along with multilocus sequence typing (MLST) and antimicrobial susceptibility testing. cdtB, cadF, and Cj0131 were present in all isolates, whereas virB11 was not detected in either domestic or travel-associated isolates. ciaB was present in all domestic isolates and 94% of travel-associated isolates. The respective detection rates of racR and wlaN in domestic and travel-associated isolates were 94% and 71% and 35.3% and 23%, respectively. MLST analyses of the 40 isolates generated 25 different sequence types (STs). ST-443 (12 isolates) and ST-21 (8 isolates) were dominant among the domestic isolates; however, STs varied among travel-associated isolates. Nalidixic acid, tetracycline, and ciprofloxacin resistance rates of the 40 isolates were 100% (40/40), 95% (38/40), and 88% (35/40), respectively. Domestic isolates exhibited 2-fold higher ciprofloxacin, telithromycin, and chloramphenicol resistance rates than travel-associated isolates. These results indicate a diverse genetic background for travel-associated C. jejuni and suggest that this pathogen may be an important emerging public health threat to travelers.
通过对7个致病基因（cdtB、cadF、Cj0131、ciaB、racR、wlaN和virB11）进行谱分析，并结合多位点序列分型（MLST）和抗菌药物敏感性测试，对40株国内和旅行相关的空肠弯曲菌分离株进行了分析。所有分离株均存在cdtB、cadF和Cj0131，而在国内或旅行相关分离株中均未检测到virB11。ciaB存在于所有国内分离株以及94%的旅行相关分离株中。racR和wlaN在国内和旅行相关分离株中的各自检测率分别为94%和71%以及35.3%和23%。对这40株分离株的MLST分析产生了25种不同的序列类型（STs）。ST-443（12株）和ST-21（8株）在国内分离株中占主导地位；然而，旅行相关分离株中的STs各不相同。这40株分离株对萘啶酸、四环素和环丙沙星的耐药率分别为100%（40/40）、95%（38/40）和88%（35/40）。国内分离株对环丙沙星、泰利霉素和氯霉素的耐药率比旅行相关分离株高2倍。这些结果表明旅行相关空肠弯曲菌具有多样的遗传背景，并提示该病原体可能是对旅行者的一种重要的新出现的公共卫生威胁。
