Zeng Dexin, Zhang Xiaoping, Xue Feng, Wang Yanhong, Jiang Luyan, Jiang Yuan
APFIC Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing, 210001, China.
Beijing Kemufeng Biopharmaceutical Co, Beijing, 102600, China.
J Food Sci. 2016 Jan;81(1):M106-13. doi: 10.1111/1750-3841.13146. Epub 2015 Nov 13.
In this study, we investigated the distribution, phenotypic and molecular typing characters of Campylobacter jejuni in domestic fowl, and livestock populations in East China, to provide some reference for researches on its molecular epidemiology. A total of 1250 samples were collected from different animal sources, and C. jejuni strains were then isolated and tested for antibiotic sensitivity. Antibiotics-resistance gene and pathogenic genes were detected by polymerase chain reaction. Phylogenic analysis on the C. jejuni strains was performed by multilocus sequence typing (MLST) method. The results showed that 108 out of the 1250 samples (mean 8.64%) were C. jejuni positive. These 108 C. jejuni strains were highly sensitive to antibiotics such as chloramphenicol, amoxicillin, amikacin, cefotaxime, and azithromycin, whereas they were highly resistant to antibiotics such as cefoperazone, cotrimoxazole, cefamandole, sulfamethoxazole, and cefradine. Pathogenicity related gene identification indicated that the mean carrying rate of adhesion related gene cadF and racR, flagellin gene flaA, toxin regulating gene cdtA, cdtB, cdtC, wlaN and virB11, heat shock proteins and transferring proteins related genes dnaJ and ceuE, CiaB and pldA were 92.45%, 38.69%, 73.58%, 71.70%, 52.83%, 96.23%, 12.26%, 1.89%, 0.94%, 65.09%, 39.62% and 9.43%, respectively. A total of 58.82% of these strains contained more than 6 pathogenicity-related genes. MLST typed 58 ST types from the 108 isolated C. jejuni strains, including 24 new types, and ST-21 was the major type, accounting for 39.3% of the total strains.
在本研究中，我们调查了华东地区家禽和家畜群体中空肠弯曲菌的分布、表型及分子分型特征，为其分子流行病学研究提供参考。共从不同动物来源采集了1250份样本，随后分离出空肠弯曲菌菌株并检测其抗生素敏感性。通过聚合酶链反应检测抗生素抗性基因和致病基因。采用多位点序列分型（MLST）方法对空肠弯曲菌菌株进行系统发育分析。结果显示，1250份样本中有108份（平均8.64%）为空肠弯曲菌阳性。这108株空肠弯曲菌菌株对氯霉素、阿莫西林、阿米卡星、头孢噻肟和阿奇霉素等抗生素高度敏感，而对头孢哌酮、复方新诺明、头孢孟多、磺胺甲恶唑和头孢拉定等抗生素高度耐药。致病性相关基因鉴定表明，黏附相关基因cadF和racR、鞭毛蛋白基因flaA、毒素调节基因cdtA、cdtB、cdtC、wlaN和virB11、热休克蛋白和转运蛋白相关基因dnaJ和ceuE、CiaB和pldA的平均携带率分别为92.45%、38.69%、73.58%、71.70%、52.83%、96.23%、12.26%、1.89%、0.94%、65.09%、39.62%和9.43%。这些菌株中共有58.82%含有6个以上致病性相关基因。MLST对108株分离的空肠弯曲菌菌株进行分型，共得到58种ST型，其中包括24种新型，ST-21为主要类型，占总菌株数的39.3%。
