Rinchik E M, Machanoff R, Cummings C C, Johnson D K
Biology Division, Oak Ridge National Laboratory, Tennessee.
Genomics. 1989 Apr;4(3):251-8. doi: 10.1016/0888-7543(89)90328-5.
Genetic analysis of radiation-induced deletion mutations involving the chromosome 7 albino (c) locus has expanded the functional map of this 6 to 11-cM region of the mouse genome. To generate one of many points of molecular access necessary for intensifying the analysis of the genes and phenotypes associated with this particular complex of deletions, we have cloned an endogenous ecotropic leukemia provirus (Emv-23), known to be closely linked to c, along with its flanking chromosome 7 sequences. A unique-sequence probe (23.3), derived from a region immediately 5' to the proviral integration site, was found to map less than 0.5 cM from c in a standard backcross analysis. Southern blot analysis of DNAs from animals carrying homozygous or overlapping albino deletions demonstrated that the 23.3 probe was deleted in several relatively small c-region deletions. The deletion mapping of the 23.3 probe places the Emv-23 locus between c and Mod-2, just proximal to a region important for male fertility and juvenile fitness. Mapping of this locus also provides a refinement of the genetic/deletion map for several mutations within this deletion complex.
对涉及7号染色体白化（c）位点的辐射诱导缺失突变进行的遗传分析，扩展了小鼠基因组中这个6到11厘摩区域的功能图谱。为了生成加强对与这种特定缺失复合体相关的基因和表型分析所需的众多分子切入点之一，我们克隆了一种内源性亲嗜性白血病原病毒（Emv-23），已知它与c紧密连锁，同时还克隆了其侧翼的7号染色体序列。在标准回交分析中，发现一个源自紧邻原病毒整合位点5'端区域的单拷贝序列探针（23.3），其与c的图距小于0.5厘摩。对携带纯合或重叠白化缺失的动物的DNA进行Southern印迹分析表明，在几个相对较小的c区域缺失中，23.3探针被删除。23.3探针的缺失定位将Emv-23位点置于c和Mod-2之间，正好位于对雄性生育力和幼年健康至关重要的区域近端。该位点的定位也对这个缺失复合体内的几个突变的遗传/缺失图谱进行了优化。
