Wu Ya-Dong, Deng Shi-Kai, Shi Chao, Zhu Jian-Chun, He Jian, Li Shun-Peng
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Life Science Laboratory Center, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):633-638. doi: 10.1099/ijs.0.068718-0. Epub 2014 Nov 19.
A Gram-reaction-negative, aerobic, rod-shaped, non-spore-forming, non-motile bacterial strain, designated BUT-6(T), was isolated from activated sludge of a wastewater-treatment facility. The strain grew at 15-35 °C (optimum 30 °C), pH 4.0-10.0 (optimum pH 7.0) and 0-3.0 % (w/v) NaCl (optimum 1.0 %). Phylogenetic analysis based on 16S rRNA sequences showed that strain BUT-6(T) was most closely related to Tahibacter aquaticus PYM5-11(T) (98.6 % similarity). However, the DNA-DNA relatedness between strain BUT-6(T) and T. aquaticus PYM5-11(T) was 47.1 %. The major fatty acids (>10 % of total fatty acids) of strain BUT-6(T) were iso-C15 : 0, iso-C17 : 1ω9c and iso-C17 : 0. The major respiratory quinone was ubiquinone Q-8. The profile of polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, an unidentified aminophospholipid, three unknown aminolipids and unidentified phospholipids. The DNA G+C content of strain BUT-6(T) was 71.7 mol%. On the basis of the data from the polyphasic taxonomic study presented, strain BUT-6(T) is considered to represent a novel species of the genus Tahibacter, for which the name Tahibacter caeni sp. nov. is proposed. The type strain is BUT-6(T) ( = CCTCC AB 2013266(T) = KACC 17139(T)).
从一家废水处理设施的活性污泥中分离出一株革兰氏阴性、需氧、杆状、不形成芽孢、无运动性的细菌菌株，命名为BUT-6(T)。该菌株在15-35 °C（最适温度30 °C）、pH 4.0-10.0（最适pH 7.0）和0-3.0%（w/v）NaCl（最适1.0%）条件下生长。基于16S rRNA序列的系统发育分析表明，菌株BUT-6(T)与水生塔希氏菌PYM5-11(T)关系最为密切（相似性为98.6%）。然而，菌株BUT-6(T)与水生塔希氏菌PYM5-11(T)之间的DNA-DNA相关性为47.1%。菌株BUT-6(T)的主要脂肪酸（占总脂肪酸的>10%）为异-C15 : 0、异-C17 : 1ω9c和异-C17 : 0。主要呼吸醌为泛醌Q-8。极性脂质谱由双磷脂酰甘油、磷脂酰甘油、磷脂酰甲基乙醇胺、一种未鉴定的氨基磷脂、三种未知氨基脂质和未鉴定的磷脂组成。菌株BUT-6(T)的DNA G+C含量为71.7 mol%。根据所呈现的多相分类研究数据，菌株BUT-6(T)被认为代表塔希氏菌属的一个新物种，为此提出名为水生塔希氏菌（Tahibacter caeni sp. nov.）的新物种。模式菌株为BUT-6(T)（= CCTCC AB 2013266(T)= KACC 17139(T)）。
