sp. nov.，分离自中国广东养猪场土壤。

sp. nov., isolated from pig farm soil in Guangdong, PR China.

机构信息

Guangdong Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China.

Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.

出版信息

Int J Syst Evol Microbiol. 2023 Apr;73(4). doi: 10.1099/ijsem.0.005852.

DOI:10.1099/ijsem.0.005852

PMID:37083489

Abstract

In the process of exploring the microbial diversity of pig farms, a Gram-stain-negative, aerobic, rod-shaped, non-spore-forming, non-motile bacterial strain, designated P2K, was isolated from soil sample collected at a pig farm, Guangdong Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P2K belonged to the genus , with the highest sequence similarity to PYM5-11 (98.6%) and BUT-6 (98.3 %). The genome size of strain P2K was 6.0 Mb with a DNA G+C content of 68.3 mol%. Average nucleotide identity values between strain P2K and the type strains of the genus were 81.1-81.6 %. The digital DNA-DNA hybridization values between P2K and these relative species were 24.5-25.6%. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, an unknown aminolipids, two unknown lipids and three unknown phospholipids. The major respiratory quinone of strain P2K was ubiquinone Q-8, and the main fatty acids (>10.0 % of total fatty acids) of strain P2K were iso-C, iso-C and summed feature 9 (C 10-methyl and/or iso-C ω9). Based on phenotypic and genotypic data, strain P2K represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain P2K (=GDMCC 1.3107=JCM 35231).

摘要

在中国广东省一个养猪场的土壤样本中，分离到一株革兰氏阴性、需氧、杆状、不产芽孢、不运动的细菌菌株，命名为 P2K。基于 16S rRNA 基因序列的系统发育分析表明，菌株 P2K 属于属，与 PYM5-11（98.6%）和 BUT-6（98.3%）的序列相似性最高。菌株 P2K 的基因组大小为 6.0 Mb，G+C 含量为 68.3 mol%。菌株 P2K 与属的模式菌株之间的平均核苷酸同一性值为 81.1-81.6%。P2K 与这些相关种之间的数字 DNA-DNA 杂交值为 24.5-25.6%。极性脂包括磷脂酰乙醇胺、磷脂酰甘油、一种未知的氨基脂、两种未知的脂和三种未知的磷脂。菌株 P2K 的主要呼吸醌是泛醌 Q-8，主要脂肪酸（占总脂肪酸的>10.0%）是 iso-C、iso-C 和总和特征 9（C 10-甲基和/或 iso-C ω9）。基于表型和基因型数据，菌株 P2K 代表属中的一个新种，建议命名为 sp. nov.，其模式菌株为 P2K（=GDMCC 1.3107=JCM 35231）。