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sp. nov.,分离自土壤;sp. nov.,分离自淡水。

sp. nov., isolated from soil and sp. nov., isolated from freshwater.

机构信息

Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.

Microbial Research Department, Nakdonggang National Institute of Biological Resources, Gyeongsangbuk-do 37242, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2023 Apr;73(4). doi: 10.1099/ijsem.0.005841.

Abstract

Two Gram-stain-negative, facultative aerobic, catalase- and oxidase-positive, and non-motile rod bacteria, strains BL and W38, that were isolated from soil and freshwater, respectively, were taxonomically characterized. Both strains optimally grew at 30 °C and pH 7.0 in Reasoner's 2A medium and contained ubiquinone-8 as the sole respiratory quinone. As major fatty acids (>10 %), strain BL contained iso-C and summed features 3 and 9 (comprising iso-C 2-OH and/or C 7/ω6 and iso-C 9 and/or C 10-methyl, respectively), whereas strain W38 contained iso-C, iso-C and summed feature 9. Diphosphatidylglycerol and phosphatidylmonomethylethanolamine as major polar lipids and phosphatidylethanolamine and phosphatidylglycerol as minor polar lipids were detected in both strains. The DNA G+C contents of strains BL and W38 were 68.3 and 65.3 %, respectively. Phylogenetic analyses based on 16S rRNA gene and genome sequences revealed that strains BL and W38 formed a tight phylogenetic lineage with species, and they shared 98.8 % 16S rRNA gene sequence similarity and 75.5 % average nucleotide identity (ANI) and 16.6 % digital DNA-DNA hybridization (dDDH) values, indicating that they are different species. Strains BL and W38 were most closely related to BUT-6 and PYM5-11 with 97.7 and 98.0 % 16S rRNA gene sequence similarities, respectively. ANI and dDDH values between strain BL and BUT-6 and between strain W38 and DSM 21667 were 78.5 and 21.6% and 75.3 and 21.0 %, respectively. Based on their phenotypic, chemotaxonomic and genomic properties, strains BL and W38 represent two different novel species of the genus , for which the names sp. nov. and sp. nov. are proposed. The type strains of and are BL (=KACC 22831=JCM 35402) and W38 (=KACC 22832=JCM 35749), respectively.

摘要

两株革兰氏阴性、兼性需氧、接触酶和氧化酶阳性、不运动的杆菌,分别命名为 BL 和 W38,从土壤和淡水分别分离得到,对其进行了分类学鉴定。两株菌在 Reasoner's 2A 培养基中最适生长温度为 30°C,pH 值为 7.0,均含有醌-8 作为唯一的呼吸醌。作为主要脂肪酸(>10%),BL 株含有 iso-C 和总和特征 3 和 9(分别包含 iso-C 2-OH 和/或 C 7/ω6 和 iso-C 9 和/或 C 10-甲基),而 W38 株含有 iso-C、iso-C 和总和特征 9。BL 和 W38 株均含有二磷脂酰甘油和单甲基乙醇胺作为主要极性脂,磷脂乙醇胺和磷脂酰甘油作为次要极性脂。BL 和 W38 株的 DNA G+C 含量分别为 68.3%和 65.3%。基于 16S rRNA 基因和基因组序列的系统发育分析表明,BL 和 W38 株与 种形成了一个紧密的系统发育分支,它们的 16S rRNA 基因序列相似性为 98.8%,平均核苷酸同一性(ANI)为 75.5%,数字 DNA-DNA 杂交(dDDH)值为 16.6%,表明它们是不同的种。BL 和 W38 株与 BUT-6 和 PYM5-11 的 16S rRNA 基因序列相似性最高,分别为 97.7%和 98.0%。BL 株与 BUT-6 和 W38 株与 DSM 21667 之间的 ANI 和 dDDH 值分别为 78.5%和 21.6%和 75.3%和 21.0%。基于表型、化学分类学和基因组特性,BL 和 W38 株代表 属的两个不同新种,分别命名为 sp. nov. 和 sp. nov.。 和 的模式菌株分别为 BL(=KACC 22831=JCM 35402)和 W38(=KACC 22832=JCM 35749)。

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