Cetera Maureen, Ramirez-San Juan Guillermina R, Oakes Patrick W, Lewellyn Lindsay, Fairchild Michael J, Tanentzapf Guy, Gardel Margaret L, Horne-Badovinac Sally
1] Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA [2] Committee on Development, Regeneration and Stem Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA.
1] Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA [2] Institute for Biophysical Dynamics, James Franck Institute and Department of Physics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
Nat Commun. 2014 Nov 21;5:5511. doi: 10.1038/ncomms6511.
Tissues use numerous mechanisms to change shape during development. The Drosophila egg chamber is an organ-like structure that elongates to form an elliptical egg. During elongation the follicular epithelial cells undergo a collective migration that causes the egg chamber to rotate within its surrounding basement membrane. Rotation coincides with the formation of a 'molecular corset', in which actin bundles in the epithelium and fibrils in the basement membrane are all aligned perpendicular to the elongation axis. Here we show that rotation plays a critical role in building the actin-based component of the corset. Rotation begins shortly after egg chamber formation and requires lamellipodial protrusions at each follicle cell's leading edge. During early stages, rotation is necessary for tissue-level actin bundle alignment, but it becomes dispensable after the basement membrane is polarized. This work highlights how collective cell migration can be used to build a polarized tissue organization for organ morphogenesis.
组织在发育过程中利用多种机制来改变形状。果蝇卵室是一种类似器官的结构，它会伸长形成椭圆形的卵。在伸长过程中，卵泡上皮细胞进行集体迁移，导致卵室在其周围的基底膜内旋转。旋转与“分子束腰”的形成同时发生，其中上皮中的肌动蛋白束和基底膜中的原纤维都与伸长轴垂直排列。在这里，我们表明旋转在构建束腰的肌动蛋白基成分中起着关键作用。旋转在卵室形成后不久开始，并且需要每个卵泡细胞前缘的片状伪足突起。在早期阶段，旋转对于组织水平的肌动蛋白束排列是必要的，但在基底膜极化后就变得不再必要。这项工作突出了集体细胞迁移如何可用于构建用于器官形态发生的极化组织组织。
