Liu Ying, Zhang Hong-wei, Zhou Rui-min, Yang Cheng-yun, Qian Dan, Zhao Yu-ling, Xu Bian-li
Henan Center for Disease Control and Prevention, Zhengzhou 450016, China.
Malar J. 2014 Nov 21;13:448. doi: 10.1186/1475-2875-13-448.
In recent years, there has been a substantial increase of imported Plasmodium vivax incidence in Henan Province. As China is in a pre-elimination phase, the surveillance of imported malaria is essential, but there is no good way to distinguish imported cases from indigenous cases. This paper reports a case of a 39-year-old man who acquired P. vivax while staying in Indonesia for one month in 2013, and relapsed in Henan, China in 2014. This was diagnosed as vivax malaria based on rapid diagnostic test, Giemsa-stained peripheral blood smear and Plasmodium species-specific nested PCR. The genetic sequence for the circumsporozoite protein genes was analysed and the genetic variations were compared with a previously constructed database of Chinese isolates. The results from the circumsporozoite protein (CSP) gene sequence analysis centered on the repeat patterns showed that the imported cases had completely different sequences from any subtypes from Chinese isolates, but well matched with the countries travelled by the patient. The imported vivax cases were able to clearly distinguish from the indigenous vivax cases by detecting the CSP gene and were able to confim its origin by genotyping.
近年来，河南省间日疟原虫输入病例的发病率大幅上升。由于中国正处于疟疾消除前阶段，输入性疟疾的监测至关重要，但目前尚无很好的方法来区分输入病例和本地病例。本文报告了一例39岁男性病例，该患者于2013年在印度尼西亚停留1个月期间感染间日疟原虫，并于2014年在中国河南复发。根据快速诊断检测、吉姆萨染色外周血涂片和疟原虫种特异性巢式PCR，该病例被诊断为间日疟。分析了环子孢子蛋白基因的遗传序列，并将遗传变异与先前构建的中国分离株数据库进行了比较。基于重复模式的环子孢子蛋白（CSP）基因序列分析结果表明，输入病例的序列与中国分离株的任何亚型完全不同，但与患者前往的国家的序列高度匹配。通过检测CSP基因，输入性间日疟病例能够与本地间日疟病例明显区分开来，并能够通过基因分型确定其来源。
