Motojima S, Yukawa T, Fukuda T, Makino S
Department of Medicine and Clinical Immunology, Dokkyo University School of Medicine, Tochigi, Japan.
Allergy. 1989 Jan;44(1):66-74. doi: 10.1111/j.1398-9995.1989.tb00448.x.
The effects of inhaled allergen on airway responsiveness and on beta- and alpha-1-adrenergic receptors on lung membrane were investigated in guinea pigs. After measuring the respiratory threshold to histamine (RT-HIS), one group of guinea pigs passively sensitized for ovalbumin was challenged by allergen inhalation (challenged group). Measurement of the RT-HIS 24 h following challenge revealed a significant decrease from 687 micrograms/ml (mean, n = 16) to 407 micrograms/ml (P less than 0.05). In addition the RT-HIS 24 h after challenge was also significantly lower in the challenged group than in controls (n = 9, P less than 0.05). The density of beta-adrenergic receptors on the lung membrane of the challenged group was 594 +/- 32 (mean +/- SE) fmol/mg protein (n = 11) compared with 712 +/- 24 fmol/mg protein (n = 9) in the controls, a statistically significant difference (P less than 0.05). A significant correlation was found between the RT-HIS and density of beta-adrenergic receptors. From these results, we concluded that the exaggerated airway responsiveness 24 h after allergen challenge is in part due to a decrease in the density of beta-adrenergic receptors. There was no difference in the density of alpha-1-adrenergic receptors nor a significant correlation between the RT-HIS and the number of alpha-1-adrenergic receptors in the challenged vs. the control groups.
在豚鼠中研究了吸入变应原对气道反应性以及肺膜上β-和α-1-肾上腺素能受体的影响。在测量对组胺的呼吸阈值（RT-HIS）后，一组对卵清蛋白被动致敏的豚鼠通过吸入变应原进行激发（激发组）。激发后24小时测量RT-HIS，结果显示其从687微克/毫升（平均值，n = 16）显著降低至407微克/毫升（P < 0.05）。此外，激发组激发后24小时的RT-HIS也显著低于对照组（n = 9，P < 0.05）。激发组肺膜上β-肾上腺素能受体的密度为594±32（平均值±标准误）飞摩尔/毫克蛋白（n = 11），而对照组为712±24飞摩尔/毫克蛋白（n = 9），差异具有统计学意义（P < 0.05）。RT-HIS与β-肾上腺素能受体密度之间存在显著相关性。从这些结果中，我们得出结论，变应原激发后24小时气道反应性增强部分是由于β-肾上腺素能受体密度降低所致。激发组与对照组相比，α-1-肾上腺素能受体密度没有差异，RT-HIS与α-1-肾上腺素能受体数量之间也没有显著相关性。
