Hayashi K, Taki F, Sugiyama S, Takagi K, Satake T, Ozawa T
Department of Internal Medicine, Faculty of Medicine, University of Nagoya, Japan.
Int Arch Allergy Appl Immunol. 1988;86(2):170-5. doi: 10.1159/000234567.
This study was designed to elucidate the mechanism responsible for alterations in the numbers of autonomic nerve receptors in experimental asthma. In the in vivo experiment, guinea pigs sensitized by exposure to aerosol of 2% ovalbumin for 7-8 min for 10 successive days were used as the experimental asthma group. The control group was exposed to saline. Beta-, alpha-1-adrenergic and muscarinic acetylcholine receptors in lung membranes were studied by direct binding techniques using l-3H-dihydroalprenolol, 3H-bunazosin and l-3H-quinuclidinyl benzilate, respectively. The experimental asthma group showed a 33% decrease in the number of beta-adrenergic receptors, a 37% increase in the number of alpha-1-adrenergic receptors and no change in the number of muscarinic acetylcholine receptors compared with the control group. The endogenous phospholipase activity was determined by high-performance liquid chromatography using tridecanoyl phosphatidylcholine as a substrate. The phospholipase activity in lung membranes in the experimental asthma group was elevated by 50% compared with that in the control group. Lung membrane phospholipid composition was analyzed by thin-layer chromatography with a flame ionization detector. In the experimental asthma group, the amounts of phosphatidylcholine and phosphatidylethanolamine decreased significantly compared with those in the control group. In the in vitro experiment after pretreatment of lung membranes with phospholipase A2, a decreased number of beta-adrenergic receptors, an increased number of alpha-1-adrenergic receptors and no change in the number of muscarinic acetylcholine receptors were observed.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究旨在阐明实验性哮喘中自主神经受体数量改变的机制。在体内实验中，连续10天每天暴露于2%卵清蛋白气雾剂7 - 8分钟致敏的豚鼠作为实验性哮喘组。对照组暴露于生理盐水。分别使用l - 3H - 二氢阿普洛尔、3H - 布那唑嗪和l - 3H - 喹核醇基苯甲酸酯，通过直接结合技术研究肺膜中的β -、α - 1 - 肾上腺素能和毒蕈碱型乙酰胆碱受体。与对照组相比，实验性哮喘组β - 肾上腺素能受体数量减少33%，α - 1 - 肾上腺素能受体数量增加37%，毒蕈碱型乙酰胆碱受体数量无变化。以内豆蔻酰磷脂酰胆碱为底物，通过高效液相色谱法测定内源性磷脂酶活性。实验性哮喘组肺膜中的磷脂酶活性比对照组升高了50%。采用带火焰离子化检测器的薄层色谱法分析肺膜磷脂组成。与对照组相比，实验性哮喘组磷脂酰胆碱和磷脂酰乙醇胺的含量显著降低。在用磷脂酶A2预处理肺膜后的体外实验中，观察到β - 肾上腺素能受体数量减少，α - 1 - 肾上腺素能受体数量增加，毒蕈碱型乙酰胆碱受体数量无变化。（摘要截短至250字）
