Xing Wanying, Li Qiang, Cao Rangjuan, Xu Zheli
Department of Breast Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
World J Surg Oncol. 2014 Nov 22;12:352. doi: 10.1186/1477-7819-12-352.
Neogenin is closely related to the human tumor suppressor gene deleted in colorectal cancer and plays a role in mammary morphogenesis. This study aimed to assess neogenin expression in breast cancer for any clinically significant association.
A total of 54 breast cancer patients who underwent modified radical mastectomy were enrolled for immunohistochemical and quantitative real-time PCR analysis of neogenin expression in their cancerous breast tissues in comparison to matching distant non-cancerous tissues.
The data showed that the neogenin protein was either strongly or moderately expressed in the cytoplasm of the distant non-cancerous cells. In contrast, neogenin protein was either weakly or not expressed in the cytoplasm of 51/54 (94.4%) breast cancer cells, among which 13 breast cancer cases did not express neogenin protein at all (13/54, 24.1%). Similarly, levels of neogenin mRNA were significantly lower in breast cancer tissues than that of the matched distant non-cancerous tissues (51/54, 94.4%). Neogenin expression was inversely associated with breast cancer grade; that is, grade III breast cancer expressed much less neogenin than grade I-II (P<0.05).
This study indicates that neogenin expression in breast cancer tissues is inversely associated with tumor grade.
Neogenin与在结直肠癌中缺失的人类肿瘤抑制基因密切相关，并在乳腺形态发生中发挥作用。本研究旨在评估乳腺癌中Neogenin的表达情况，以确定是否存在任何具有临床意义的关联。
共有54例行改良根治性乳房切除术的乳腺癌患者纳入研究，对其癌性乳腺组织及配对的远处非癌组织进行Neogenin表达的免疫组织化学和定量实时PCR分析。
数据显示，Neogenin蛋白在远处非癌细胞的细胞质中呈强或中度表达。相比之下，51/54（94.4%）乳腺癌细胞的细胞质中Neogenin蛋白呈弱表达或不表达，其中13例乳腺癌病例完全不表达Neogenin蛋白（13/54，24.1%）。同样，乳腺癌组织中Neogenin mRNA水平显著低于配对的远处非癌组织（51/54，94.4%）。Neogenin表达与乳腺癌分级呈负相关；即III级乳腺癌的Neogenin表达远低于I-II级（P<0.05）。
本研究表明，乳腺癌组织中Neogenin的表达与肿瘤分级呈负相关。
