Lindbäck B, Bergman A
Department of Clinical Chemistry, Huddinge University Hospital, Sweden.
Clin Chem. 1989 May;35(5):835-7.
We evaluated a new, simple, enzymatic kinetic method from Wako Chemicals GmbH in comparison with a kinetic Jaffé method by using isotope dilution-mass spectrometry (ID-MS) as a reference method. An ID-MS-calibrated serum standard was used. Both the enzymatic and the Jaffé method correlated well with ID-MS, except for sera with high concentrations of bilirubin. Ethyl acetoacetate, acetone, and glucose in serum interfered somewhat with the Jaffé method but not with the enzymatic method. We conclude that the present enzymatic method has merit as compared with a Jaffé method for routine work, but is more expensive.
我们评估了来自德国和光纯药工业株式会社的一种新的、简单的酶动力学方法，并将其与动力学Jaffé法进行比较，以同位素稀释质谱法（ID-MS）作为参考方法。使用了经ID-MS校准的血清标准品。除了高胆红素浓度的血清外，酶法和Jaffé法与ID-MS的相关性都很好。血清中的乙酰乙酸乙酯、丙酮和葡萄糖对Jaffé法有一定干扰，但对酶法没有干扰。我们得出结论，与Jaffé法相比，目前的酶法在常规工作中具有优点，但成本更高。
