Sugita O, Uchiyama K, Yamada T, Sato T, Okada M, Takeuchi K
Department of Laboratory Medicine, Niigata University School of Medicine, Japan.
Ann Clin Biochem. 1992 Sep;29 ( Pt 5):523-8. doi: 10.1177/000456329202900506.
A new, totally enzymatic procedure for the determination of creatinine in serum and urine, using creatinine amidohydrolase, creatine amidinohydrolase, sarcosine oxidase and formaldehyde dehydrogenase is described. The assay was adapted to a discontinuous analyser with each analysis requiring only 20 microL of serum or 3 microL of urine. Analytical recovery of creatinine in serum and urine averaged 100.6%. Within-run and between-run precision studies gave coefficients of variation of 1.1% and 1.8%, respectively, for a serum with mean values of 83 mumol/L (9.4 mg/L) creatinine. Creatinine concentrations in serum and urine were measured by this procedure, in Japanese children and adults. The reference intervals for serum creatinine concentrations in adults were 55-96 mumol/L (6.2-10.9 mg/L) in men and 40-66 mumol/L (4.5-7.5 mg/L) in women, and for urine, 9.46-19.01 mmol/day (1070-2150 mg/day) in men and 6.75-10.61 mmol/day (764-1200 mg/day) in women. The reference intervals of creatinine clearance were 88.0-176.4 mL/min in men and 75.7-173.0 mL/min in women.
本文描述了一种全新的、完全酶促的血清和尿液肌酐测定方法，该方法使用肌酐酰胺水解酶、肌酸脒基水解酶、肌氨酸氧化酶和甲醛脱氢酶。该检测方法适用于间断分析仪，每次分析仅需20微升血清或3微升尿液。血清和尿液中肌酐的分析回收率平均为100.6%。对于肌酐平均值为83微摩尔/升（9.4毫克/升）的血清，批内和批间精密度研究的变异系数分别为1.1%和1.8%。采用此方法对日本儿童和成人的血清及尿液中的肌酐浓度进行了测定。成人血清肌酐浓度的参考区间为：男性55 - 96微摩尔/升（6.2 - 10.9毫克/升），女性40 - 66微摩尔/升（4.5 - 7.5毫克/升）；尿液中肌酐浓度的参考区间为：男性9.46 - 19.01毫摩尔/天（1070 - 2150毫克/天），女性6.75 - 10.61毫摩尔/天（764 - 1200毫克/天）。肌酐清除率的参考区间为：男性88.0 - 176.4毫升/分钟，女性75.7 - 173.0毫升/分钟。
