Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.
Stem Cell Rev Rep. 2015 Apr;11(2):288-97. doi: 10.1007/s12015-014-9576-2.
It was recently shown that the conditioned media (CM) of Human Umbilical Cord Perivascular Cells (HUCPVCs), a mesenchymal progenitor population residing within the Wharton Jelly of the umbilical cord, was able to modulate in vitro the survival and viability of different neuronal and glial cells populations. In the present work, we aimed to assess if the secretome of HUCPVCs is able to 1) induce the differentiation of human telencephalon neural precursor cells (htNPCs) in vitro, and 2) modulate neural/glial proliferation, differentiation and survival in the dentate gyrus (DG) of adult rat hippocampus. For this purpose, two separate experimental setups were performed: 1) htNPCs were incubated with HUCPVCs-CM for 5 days after which neuronal differentiation was assessed and, 2) HUCPVCs, or their respective CM, were injected into the DG of young adult rats and their effects assessed 7 days later. Results revealed that the secretome of HUCPVCs was able to increase neuronal cell differentiation in vitro; indeed, higher densities of immature (DCX(+) cells) and mature neurons (MAP-2(+) cells) were observed when htNPCs were incubated with the HUCPVCs-CM. Additionally, when HUCPVCs and their CM were injected in the DG, results revealed that both cells or CM were able to increase the endogenous proliferation (BrdU(+) cells) 7 days after injection. It was also possible to observe an increased number of newborn neurons (DCX(+) cells), upon injection of HUCPVCs or their respective CM. Finally western blot analysis revealed that after CM or HUCPVCs transplantation, there was an increase of fibroblast growth factor-2 (FGF-2) and, to a lesser extent, of nerve growth factor (NGF) in the DG tissue. Concluding, our results have shown that the transplantation of HUCPVCs or the administration of their secretome were able to potentiate neuronal survival and differentiation in vitro and in vivo.
最近的研究表明，人脐带血管周细胞（HUCPVCs）的条件培养基（CM）能够调节不同神经元和神经胶质细胞群体的体外存活和活力，HUCPVCs 是一种存在于脐带华通胶中的间充质祖细胞群体。在本研究中，我们旨在评估 HUCPVCs 的分泌组是否能够 1）在体外诱导人端脑神经前体细胞（htNPCs）的分化，以及 2）调节成年大鼠海马齿状回（DG）中的神经/神经胶质增殖、分化和存活。为此，我们进行了两个独立的实验设置：1）将 htNPCs 与 HUCPVCs-CM 孵育 5 天，然后评估神经元分化情况，以及 2）将 HUCPVCs 或其相应的 CM 注射到年轻成年大鼠的 DG 中，并在 7 天后评估其效果。结果表明，HUCPVCs 的分泌组能够增加体外神经元细胞的分化；事实上，当 htNPCs 与 HUCPVCs-CM 孵育时，观察到更多未成熟（DCX（+）细胞）和成熟神经元（MAP-2（+）细胞）的密度增加。此外，当 HUCPVCs 及其 CM 注射到 DG 中时，结果表明，细胞或 CM 均能在注射后 7 天增加内源性增殖（BrdU（+）细胞）。此外，注射 HUCPVCs 或其相应 CM 后，也观察到新生神经元（DCX（+）细胞）数量增加。最后，Western blot 分析显示，在 CM 或 HUCPVCs 移植后，DG 组织中纤维母细胞生长因子-2（FGF-2）的含量增加，而神经生长因子（NGF）的含量则略有增加。综上所述，我们的研究结果表明，HUCPVCs 的移植或其分泌组的给药能够增强体外和体内神经元的存活和分化。
