DcR3在膀胱尿路上皮癌组织中的过表达及其临床病理意义
Overexpression and clinicopathological contribution of DcR3 in bladder urothelial carcinoma tissues.
作者信息
Jiang Yi-Qiang, Zhong Teng-Fei, Dang Yi-Wu, Zou Ling-Song, Yang Liu, Yang Xia, Chen Gang
机构信息
Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, China E-mail :
出版信息
Asian Pac J Cancer Prev. 2014;15(21):9137-42. doi: 10.7314/apjcp.2014.15.21.9137.
BACKGROUND
To explore the expression of DcR3 protein and its clinicopathological significance in bladder urothelial carcinomas (BUC).
MATERIALS AND METHODS
Immunohistochemistry was performed to detect the expression of DcR3, caspase-3, Bcl-2, VEGF, Ki-67, PCNA and P53 in 166 BUC and 56 normal bladder tissues. Western blotting was used to detect the expression of DcR3 in the supernatants of cultured BUC cells.
RESULTS
Overexpression of DcR3 was found in BUC tissues and cell lines, with significant elevation as compared to normal bladder tissues (p<0.0001). Higher DcR3 expression was related to the status of invasion, lymph node metastasis and recurrence. Furthermore, DcR3 expression was negatively correlated with caspase-3 and positively associated with Bcl-2, VEGF, Ki-67 labeling index (LI), PCNA LI and P53 (all p<0.0001), respectively.
CONCLUSIONS
DcR3 may play a crucial role as an oncogene in tumorigenesis, deterioration and progress of BUC via influencing related pathways of apoptosis, proliferation and angiogenesis. The detection of DcR3 protein in the formalin- fixed and paraffin-embedded samples could assist to predict in prognosis of BUC patients.
背景
探讨DcR3蛋白在膀胱尿路上皮癌（BUC）中的表达及其临床病理意义。
材料与方法
采用免疫组织化学法检测166例BUC组织和56例正常膀胱组织中DcR3、半胱天冬酶-3、Bcl-2、血管内皮生长因子（VEGF）、Ki-67、增殖细胞核抗原（PCNA）和P53的表达。采用蛋白质印迹法检测培养的BUC细胞上清液中DcR3的表达。
结果
在BUC组织和细胞系中发现DcR3过表达，与正常膀胱组织相比显著升高（p<0.0001）。较高的DcR3表达与浸润状态、淋巴结转移和复发有关。此外，DcR3表达分别与半胱天冬酶-3呈负相关，与Bcl-2、VEGF、Ki-67标记指数（LI）、PCNA LI和P53呈正相关（均p<0.0001）。
结论
DcR3可能通过影响凋亡、增殖和血管生成的相关途径，在BUC的肿瘤发生、恶化和进展中作为癌基因发挥关键作用。检测福尔马林固定石蜡包埋样本中的DcR3蛋白有助于预测BUC患者的预后。
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