费氏弧菌luxR基因在大肠杆菌中转录负自调控对自诱导物的需求
Requirement for autoinducer in transcriptional negative autoregulation of the Vibrio fischeri luxR gene in Escherichia coli.
作者信息
Dunlap P V, Ray J M
机构信息
Department of Biology, New Mexico State University, Las Cruces 88003.
出版信息
J Bacteriol. 1989 Jun;171(6):3549-52. doi: 10.1128/jb.171.6.3549-3552.1989.
Abstract
The effect of a mutation in luxI (autoinducer synthetase gene) on transcription of luxR in the cloned Vibrio fischeri lux system (luxR, luxICDABE) was examined in Escherichia coli. For the luxI mutant, transcription from the luxR promoter (monitored with beta-galactosidase levels from a luxR::lacZ fusion, with LuxR supplied in trans) decreased fivefold, to levels of the luxI+ strain, only in the presence of added autoinducer. The results demonstrate that, as has been shown at the translational level, autoinducer is required for negative autoregulation of luxR at the transcriptional level.
摘要
在大肠杆菌中检测了luxI（自诱导物合成酶基因）突变对克隆的费氏弧菌lux系统（luxR、luxICDABE）中luxR转录的影响。对于luxI突变体，仅在添加自诱导物的情况下，luxR启动子的转录（通过luxR::lacZ融合体的β-半乳糖苷酶水平监测，LuxR通过反式提供）下降了五倍，降至luxI+菌株的水平。结果表明，如在翻译水平上所显示的那样，自诱导物是luxR在转录水平上进行负自调控所必需的。
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