Li Guiying, Zhang Xinbo, Wang Zhiwen, Shi Ying, Chen Tao, Zhao Xueming
Sheng Wu Gong Cheng Xue Bao. 2014 Aug;30(8):1151-63.
In the last few years, high-throughput (or 'next-generation') sequencing technologies have delivered a step change in our ability to sequence genomes, whether human or bacterial. Further comparative genome analysis enables us to reveal detailed knowledge of genetics or physiology of industrial important strains obtained in laboratory, to analyze genotype-phenotype correlations of mutants with improved performance. Based on identified key mutations or mutation combinations, Inverse Metabolic Engineering (IME) can be performed by using accurate genetic modification system. Recently, IME has been successfully used for strain improvement and has become a research hotspot, including improving substrate utilization, engineering the robustness of industrial microbes and enhancing production of bio-based products. Here, we describe recent advances in research methods of IME, with an emphasis on characterization of genotype-phenotype and the latest advances and application of IME. Possible directions and challenges for further development of IME are also discussed.
在过去几年中，高通量（或“新一代”）测序技术使我们在对基因组进行测序方面有了质的飞跃，无论是人类基因组还是细菌基因组。进一步的比较基因组分析使我们能够揭示在实验室获得的具有工业重要性的菌株的遗传学或生理学详细知识，分析性能改进的突变体的基因型-表型相关性。基于已鉴定的关键突变或突变组合，可以使用精确的基因编辑系统进行逆向代谢工程（IME）。最近，IME已成功用于菌株改良，并成为一个研究热点，包括提高底物利用率、改造工业微生物的鲁棒性以及提高生物基产品的产量。在此，我们描述了IME研究方法的最新进展，重点是基因型-表型的表征以及IME的最新进展和应用。还讨论了IME进一步发展的可能方向和挑战。
