Kucharská Z, Danes L, Dobahi S S, Kopecký K, Svandová E
Institute of Hygiene and Epidemiology, Prague, Czechoslovakia.
J Hyg Epidemiol Microbiol Immunol. 1989;33(1):75-82.
Levels of complement-fixing antibodies against rotaviruses were evaluated in the sera of 900 healthy children aged 1-9 years 300 sera were collected in the People's Democratic Republic of Yemen in September-October 1985, 300 sera were obtained in the Czech Socialist Republic in the same period and another 300 also in the Czech Socialist Republic in September-October 1986. The latter two groups were investigated in the framework of immunological surveys. A complement-fixation antigen was prepared from a simian strain of the rotavirus type SA-11 in a tissue cell line MA-104. The sera from Yemen featured lower mean titres in the age groups and thus the lowest overall titre. As the antibody titre increased, the portion of seropositive sera from Yemen declined by far more rapidly than in the Czech children, where it remained virtually the same. The sera from Yemen showed the lowest negative rate and lowest ratio of high titres. The antibody titre of 1:64 and higher was not detected in children from Yemen, while they occurred in the two groups of Czech children. There was no correlation between antibody titres and probands' sex, nor was there linear dependence of titre magnitude on age. The mean positivity rate in each group as assessed by the antibody titres was the lowest in the sera from Yemen. The percentage of positive sera in all age groups was higher in the Czech children with the exception of children from Yemen aged 6 and 9 years. The aim of the present study was to evaluate the antibody status in infant populations and thus expand knowledge of rotavirus epidemiology.
对900名1至9岁健康儿童的血清进行了抗轮状病毒补体结合抗体水平评估。1985年9月至10月在也门民主人民共和国采集了300份血清，同期在捷克斯洛伐克社会主义共和国获得了300份血清，1986年9月至10月又在捷克斯洛伐克社会主义共和国采集了另外300份血清。后两组是在免疫调查框架内进行研究的。用轮状病毒SA - 11型猿猴株在MA - 104组织细胞系中制备补体结合抗原。也门的血清在各年龄组中的平均滴度较低，因此总体滴度最低。随着抗体滴度升高，也门血清阳性血清的比例下降速度远比捷克儿童快，捷克儿童的该比例几乎保持不变。也门的血清显示出最低的阴性率和高滴度比例。也门儿童中未检测到滴度为1:64及以上的抗体，而在两组捷克儿童中出现了这种情况。抗体滴度与被试者性别之间无相关性，滴度大小与年龄也无线性依赖关系。根据抗体滴度评估，每组中的平均阳性率在也门血清中最低。除了也门6岁和9岁的儿童外，捷克儿童在所有年龄组中的阳性血清百分比都更高。本研究的目的是评估婴儿群体中的抗体状况，从而扩展对轮状病毒流行病学的认识。
